Lactate and Amino Acid Catabolism in the Cheese-Ripening Yeast Yarrowia lipolytica
Lactate and Amino Acid Catabolism in the Cheese-Ripening Yeast Yarrowia lipolytica
复制标题
DOI：
10.1128/aem.01519-08
复制
发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Bonnarme, P.
中科院分区：
文献类型：
--
作者：
Mansour, S.;Beckerich, J. M.;Bonnarme, P.
乳酸和氨基酸的消耗对于干酪成熟过程中微生物的发育和/或香气的产生是非常重要的。从奶酪中分离的解脂耶氏酵母菌株在含有乳酸盐的液体培养基中在低（0.1 X）或高（2 X）浓度的氨基酸存在下生长。我们的研究结果表明，有一个显着的增长，Y。解脂菌在含有高氨基酸浓度的培养基中，但乳酸消耗有限。相反，在氨基酸耗尽完成后，乳酸在含有低浓度氨基酸的培养基中被有效消耗。这些数据表明氨基酸被Y.脂肪分解作为主要能量来源，而乳酸在氨基酸耗尽后被消耗。氨基酸降解伴随着氨的产生，相应于pH值的急剧增加。还研究了在乳酸盐上生长的解脂菌培养物。用特异性引物对5个参与氨基酸转运和催化的基因进行实时定量PCR分析，包括氨基酸转运蛋白基因（GAP 1）和4个氨基转移酶基因（ARO 8、ARO 9、BAT 1和BAT 2）。还研究了参与乳酸转运和催化的三个基因的表达。这些基因包括一个乳酸转运蛋白基因（JEN 1）和两个乳酸脱氢酶基因（CYB 2 -1和CYB 2 -2）。我们的数据显示，GAP 1、BAT 2、BAT 1和ARO 8在添加氨基酸后15至30分钟后最大表达（BAT 2是最高表达的基因），而JEN 1、CYB 2 -1和CYB 2 -2的最大表达被延迟（>= 60分钟）。
The consumption of lactate and amino acids is very important for microbial development and/or aroma production during cheese ripening. A strain of Yarrowia lipolytica isolated from cheese was grown in a liquid medium containing lactate in the presence of a low (0.1 x) or high (2 x) concentration of amino acids. Our results show that there was a dramatic increase in the growth of Y. lipolytica in the medium containing a high amino acid concentration, but there was limited lactate consumption. Conversely, lactate was efficiently consumed in the medium containing a low concentration of amino acids after amino acid depletion was complete. These data suggest that the amino acids are used by Y. lipolytica as a main energy source, whereas lactate is consumed following amino acid depletion. Amino acid degradation was accompanied by ammonia production corresponding to a dramatic increase in the pH. The effect of adding amino acids to a Y. lipolytica culture grown on lactate was also investigated. Real-time quantitative PCR analyses were performed with specific primers for five genes involved in amino acid transport and catabolism, including an amino acid transporter gene (GAP1) and four aminotransferase genes (ARO8, ARO9, BAT1, and BAT2). The expression of three genes involved in lactate transport and catabolism was also studied. These genes included a lactate transporter gene (JEN1) and two lactate dehydrogenase genes (CYB2-1 and CYB2-2). Our data showed that GAP1, BAT2, BAT1, and ARO8 were maximally expressed after 15 to 30 min following addition of amino acids (BAT2 was the most highly expressed gene), while the maximum expression of JEN1, CYB2-1, and CYB2-2 was delayed (>= 60 min).