EXPRESSION OF CHIMAERIC GENES TRANSFERRED INTO PLANT-CELLS USING A TI-PLASMID-DERIVED VECTOR
EXPRESSION OF CHIMAERIC GENES TRANSFERRED INTO PLANT-CELLS USING A TI-PLASMID-DERIVED VECTOR
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DOI：
10.1038/303209a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHELL, J
中科院分区：
文献类型：
--
作者：
HERRERAESTRELLA, L;DEPICKER, A;SCHELL, J
用Ti质粒载体导入植物细胞的外源基因不表达。从胭脂碱合成酶的启动子序列衍生表达载体，并将章鱼碱合成酶基因和氯霉素乙酰转移酶基因的编码序列插入该载体中。这些嵌合基因在通过根癌农杆菌Ti质粒转移后在植物细胞中功能性表达。
Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. An expression vector was derived from the promoter sequence of nopaline synthase, and the coding sequences of the octopine synthase gene and a chloramphenical acetyltransferase gene were inserted into this vector. These chimeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.