Connexin Expression in Renin-Producing Cells
Connexin Expression in Renin-Producing Cells
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DOI：
10.1681/asn.2008030252
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发表时间：
2009-03-01
影响因子：
13.6
通讯作者：
Wagner, Charlotte
中科院分区：
文献类型：
--
作者：
Kurtz, Lisa;Janssen-Bienhold, Ulrike;Wagner, Charlotte
连接蛋白40（Cx40）的缺乏导致异位肾小球肾素表达，并废除募集的肾素表达细胞在成人肾脏，但不干扰肾脏发育过程中的肾素表达。为了解释这些观察结果，我们的目的是分析在正常肾小球上皮样细胞，在招募的肾素表达细胞，和胎儿的肾素表达细胞的主要血管Cx的表达模式。我们发现，在肾脏发育过程中，产生肾素的细胞的出现抑制了Cx40的表达，在较小程度上，抑制了Cx45的表达，但没有抑制其他Cxs的表达。在成人肾脏，肾小球上皮样细胞表达Cx40和少量的Cx 37和Cx43，但不Cx45，定位于小动脉平滑肌细胞。在成年肾脏中，对长期盐剥夺小鼠的反应中产生肾素的细胞的募集仅与Cx40的再现相关。Cx40-null肾素产生细胞不表达Cx 37、Cx43或Cx45。这些发现表明，Cx40表达是肾脏中的肾素产生细胞的特征，并且它似乎在成人而不是胎儿肾脏中的肾素产生细胞的募集中是必不可少的。
Absence of connexin 40 (Cx40) leads to ectopic juxtaglomerular renin expression and abrogates recruitment of renin-expressing cells in the adult kidney but does not disturb renin expression during kidney development. To find an explanation for these observations, we aimed to analyze the expression pattern of major vascular Cxs in normal juxtaglomerular epithelioid cells, in recruited renin-expressing cells, and in fetal renin-expressing cells. We found that during kidney development, the appearance of renin-producing cells paralleled the expression of Cx40 and, to a lesser extent, Cx45 but not other Cxs. In the adult kidney, juxtaglomerular epithelioid cells expressed Cx40 and lesser amounts of Cx37 and Cx43 but not Cx45, which localized to arteriolar smooth muscle cells. Recruitment of renin-producing cells in adult kidneys in response to long-term salt deprivation of mice correlated with the reappearance of only Cx40. Cx40-null renin-producing cells did not express Cx37, Cx43, or Cx45. These findings suggest that Cx40 expression is a characteristic of renin-producing cells in the kidney, and it seems to be essential in the recruitment of renin-producing cells in the adult but not the fetal kidney.