Structure and biophysics of type III secretion in bacteria.
Structure and biophysics of type III secretion in bacteria.
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DOI：
10.1021/bi400160a
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发表时间：
2013-04-16
期刊：
影响因子：
2.9
通讯作者：
De Guzman, Roberto N.
中科院分区：
文献类型：
--
作者：
Chatterjee, Srirupa;Chaudhury, Sukanya;McShan, Andrew C.;Kaur, Kawaljit;De Guzman, Roberto N.
许多植物和动物细菌病原体组装一个针状纳米机器，III型分泌系统（T3SS），将毒力蛋白直接注入真核细胞以启动感染。细菌将效应子注射到宿主细胞中的能力对于许多革兰氏阴性菌的感染、存活和发病是必不可少的，所述革兰氏阴性菌包括沙门氏菌、埃希氏菌、志贺氏菌、耶尔森氏菌、假单胞菌和衣原体属。这些病原体是造成各种疾病的原因，如伤寒、大规模食源性疾病、痢疾、淋巴腺鼠疫、二级医院感染和性传播疾病。T3SS由结构蛋白和非结构蛋白组成。结构蛋白组装针器，其由膜嵌入的基底结构、从细菌表面突出的外部针和覆盖针的尖端复合物组成。在宿主细胞接触时，在针尖复合物和宿主细胞之间组装易位子，通过在宿主细胞膜中产生孔而充当效应蛋白易位的通道。在递送到宿主细胞质中之后，效应物通过操纵宿主细胞生物学（例如细胞信号传导、分泌运输、细胞骨架动力学和炎症反应）来启动和维持感染。最后，分子伴侣通过隔离细菌细胞质内的效应物和一些结构蛋白来作为分泌的调节剂。本文将重点介绍有关针器结构和生物物理学的最新进展和未来的挑战。
Many plant and animal bacterial pathogens assemble a needle-like nanomachine, the type III secretion system (T3SS), to inject virulence proteins directly into eukaryotic cells to initiate infection. The ability of bacteria to inject effectors into host cells is essential for infection, survival, and pathogenesis for many Gram-negative bacteria, including Salmonella, Escherichia, Shigella, Yersinia, Pseudomonas, and Chlamydia spp. These pathogens are responsible for a wide variety of diseases, such as typhoid fever, large-scale food-borne illnesses, dysentery, bubonic plague, secondary hospital infections, and sexually transmitted diseases. The T3SS consists of structural and nonstructural proteins. The structural proteins assemble the needle apparatus, which consists of a membrane-embedded basal structure, an external needle that protrudes from the bacterial surface, and a tip complex that caps the needle. Upon host cell contact, a translocon is assembled between the needle tip complex and the host cell, serving as a gateway for translocation of effector proteins by creating a pore in the host cell membrane. Following delivery into the host cytoplasm, effectors initiate and maintain infection by manipulating host cell biology, such as cell signaling, secretory trafficking, cytoskeletal dynamics, and the inflammatory response. Finally, chaperones serve as regulators of secretion by sequestering effectors and some structural proteins within the bacterial cytoplasm. This review will focus on the latest developments and future challenges concerning the structure and biophysics of the needle apparatus.
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DOI：
10.1083/jcb.147.3.683
发表时间：
1999-11-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blocker A;Gounon P;Larquet E;Niebuhr K;Cabiaux V;Parsot C;Sansonetti P
通讯作者：
Sansonetti P
影响因子：
3.6
作者：
Deane JE;Graham SC;Mitchell EP;Flot D;Johnson S;Lea SM
通讯作者：
Lea SM
影响因子：
8
作者：
Chatterjee, Srirupa;Zhong, Dalian;De Guzman, Roberto N.
通讯作者：
De Guzman, Roberto N.
影响因子：
5.7
作者：
Derewenda, U;Mateja, A;Waugh, DS
通讯作者：
Waugh, DS
影响因子：
3.6
作者：
Crepin, VF;Prasannan, S;Matthews, S
通讯作者：
Matthews, S