Differences in Surface Marker Expression and Chondrogenic Potential among Various Tissue-Derived Mesenchymal Cells from Elderly Patients with Osteoarthritis
Differences in Surface Marker Expression and Chondrogenic Potential among Various Tissue-Derived Mesenchymal Cells from Elderly Patients with Osteoarthritis
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DOI：
10.1159/000334400
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Jose Martinez-Lorenzo, Maria
中科院分区：
文献类型：
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作者：
Alegre-Aguaron, Elena;Desportes, Paula;Jose Martinez-Lorenzo, Maria
间充质干细胞（MSC）是自我更新的多能细胞，可用于修复骨关节炎（OA）等疾病中受损的软骨。在这项研究中，我们使用骨髓，脂肪组织关节和皮下位置，滑液样本从18例膝关节骨性关节炎，以找到一个合适的替代来源的分离间充质干细胞具有高软骨形成潜力。所有分析组织的间充质干细胞具有成纤维细胞形态，但其增殖率各不相同。皮下脂肪来源的MSC比骨髓和Hoffa脂肪垫来源的MSC增殖更快，而滑液来源的MSC生长更慢。在所有MSC组中，CD36和CD54表达相似，具有几个微小差异。这些表面标志物在皮下脂肪来源的MSC中的高表达与向透明软骨分化不良相关。滑膜液来源的MSC呈现出相对较小的成软骨分化能力，而Hoffa脂肪垫来源的MSC具有较强的成软骨潜力。总之，来自老年OA患者的MSC可能仍然显示出显着的软骨形成潜力，这取决于它们的来源。版权所有（C）2012 S. Karger AG，巴塞尔
Mesenchymal stem cells (MSCs) are self-renewing, multipotent cells that could potentially be used to repair injured cartilage in diseases such as osteoarthritis (OA). In this study we used bone marrow, adipose tissue from articular and subcutaneous locations, and synovial fluid samples from 18 patients with knee OA to find a suitable alternative source for the isolation of MSCs with high chondrogenic potential. MSCs from all tissues analysed had a fibroblastic morphology, but their rates of proliferation varied. Subcutaneous fat-derived MSCs proliferated faster than bone marrow- and Hoffa's fat pad-derived MSCs, while synovial fluid-derived MSCs grew more slowly. CD36 and CD54 expression was similar across all groups of MSCs with several minor differences. High expression of these surface markers in subcutaneous fat-derived MSCs was correlated with poor differentiation into hyaline cartilage. Synovial fluid-derived MSCs presented a relatively small chondrogenic differentiation capacity while Hoffa's fat pad-derived MSCs had strong chondrogenic potential. In conclusion, MSCs from elderly patients with OA may still display significant chondrogenic potential, depending on their origin. Copyright (C) 2012 S. Karger AG, Basel