Lotus-leaf-like topography predominates over adsorbed ECM proteins in poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) surface/cell interactions.
Lotus-leaf-like topography predominates over adsorbed ECM proteins in poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) surface/cell interactions.
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DOI：
10.1021/am4017329
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发表时间：
2013-06
影响因子：
9.5
通讯作者：
Jun Zheng;Dan Li;Lin Yuan;Xiaoli Liu;Hong Chen
中科院分区：
文献类型：
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作者：
Jun Zheng;Dan Li;Lin Yuan;Xiaoli Liu;Hong Chen
众所周知，细胞外基质(ECM)蛋白介导细胞/表面的相互作用。然而，引入特定的表面形貌可能会扰乱细胞外基质蛋白在给定表面上的吸附和细胞黏附之间的相关性。在可生物降解材料聚(3-羟基丁酸酯-3-羟基己酸酯)(PHBHHx)表面引入了荷叶状形貌。研究了蛋白质在荷叶状表面(命名为PHBHHx-L)上的吸附和细胞相互作用。水接触角数据表明，荷叶状形貌的引入增强了PHBHHx的疏水性。采用酶联免疫吸附试验检测细胞外基质蛋白(纤维粘连蛋白和玻璃体连接蛋白)在PHBHHx-L上的吸附情况。与平坦的PHBHHx相比，PHBHHx-L表面对纤维粘连蛋白和玻璃体粘连蛋白的吸附分别增加了约260%和40%。相比之下，成纤维细胞和内皮细胞在PHBHHx-L表面的黏附和增殖比在平坦聚合物表面上的黏附和增殖减少。这些结果表明，荷叶状形貌对细胞黏附和增殖的抑制作用优于吸附的黏附蛋白对黏附和增殖的促进作用。结果表明，在细胞/PHBHHx-L相互作用中，荷叶状形貌起主导作用。目前的发现表明，表面形貌、吸附蛋白质和细胞-表面相互作用之间的相互作用是复杂的。
It is well-known that extracellular matrix (ECM) proteins mediate cell/surface interactions. However, introduction of a specific surface topography may disturb the correlation between ECM proteins adsorption and cells adhesion on a given surface. In present study, lotus-leaf-like topography was introduced on the surface of a biodegradable material, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Protein adsorption and cell interactions with this lotus-leaf-like surface (designated PHBHHx-L) were investigated. Water contact angle data indicated that the hydrophobicity of PHBHHx was enhanced by the introduction of lotus-leaf-like topography. The adsorption of extracellular matrix proteins (fibronectin and vitronectin) on PHBHHx-L was measured by enzyme linked immunosorbent assay (ELISA). Compared with flat PHBHHx, adsorption on the PHBHHx-L surface increased by ~260% for fibronectin and ~40% for vitronectin. In contrast, fibroblast and endothelial cell adhesion and proliferation were reduced on the PHBHHx-L compared to the flat polymer surface. These results suggest that the inhibition of cell adhesion and proliferation caused by the lotus-leaf-like topography dominates over the effect of the adsorbed adhesive proteins in promoting adhesion and proliferation. It can be concluded that the lotus-leaf-like topography plays a dominant role in cell/PHBHHx-L interactions. The present findings indicate the complexity of the interplay among surface topography, adsorbed proteins, and cell-surface interactions.