Open Access Research Article Transcriptional Regulation of the Crk/duf26 Group of Receptor-like Protein Kinases by Ozone and Plant Hormones in Arabidopsis
Open Access Research Article Transcriptional Regulation of the Crk/duf26 Group of Receptor-like Protein Kinases by Ozone and Plant Hormones in Arabidopsis
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通讯作者：
Michael Wrzaczek;M. Brosché;J. Salojärvi;Saijaliisa Kangasjärvi;N. Idänheimo;Sophia Mersmann;S. Robatzek-S.-Robatz
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作者：
Michael Wrzaczek;M. Brosché;J. Salojärvi;Saijaliisa Kangasjärvi;N. Idänheimo;Sophia Mersmann;S. Robatzek-S.-Robatz
背景：植物受体样/Pelle 激酶 (RLK) 是一组保守的信号成分，可调节发育程序以及对生物和非生物胁迫的反应。最大的 RLK 群之一由未知功能域 26 (DUF26) RLK 组成，也称为富含半胱氨酸的受体样激酶 (CRK)，已被认为在病原体防御和程序性细胞死亡的调节中发挥重要作用。然而，尽管植物中存在大量 RLK，但只有少数 RLK 得到了功能表征。结果：我们检查了臭氧 (O 3)、强光和病原体/激发子处理条件对所有拟南芥 CRK 的转录调节，这些条件已知可诱导各种亚细胞区室中活性氧 (ROS) 的产生。一些 CRK 是通过暴露于 O 3 而不是通过光胁迫来转录诱导的。 O 3 诱导细胞外氧化爆发，而光应激则导致叶绿体中产生 ROS。对公开的微阵列数据的分析表明，CRK 对 O 3 的转录反应与对微生物或病原体相关分子模式 (PAMP) 的反应非常相似。几种激素生物合成或信号传导发生改变的突变体显示出基础转录反应和O 3 诱导的转录反应的变化。结论：将多种处理的表达分析与激素生物合成或信号传导改变的突变体相结合，提出了一个模型，其中 O 3 和水杨酸 (SA) 激活表现出负串扰的单独信号传导途径。尽管 O 3 被归类为植物的非生物胁迫，但 CRK 的转录谱显示 O 3 和生物胁迫反应之间有很强的相似性。背景 受体样/Pelle 激酶 (RLK) 是植物发育调节、激素信号传导、非生物和生物胁迫反应的重要组成部分。 RLK 是丝氨酸-苏氨酸蛋白激酶，通常包含信号肽、可变胞外结构域、跨膜区和保守的胞内蛋白激酶结构域。细胞外配体结合结构域感知信号，通常用于将 RLK 分为不同的亚组 [1]。 RLK 是拟南芥中最大的基因家族之一，拥有超过
Background: Plant Receptor-like/Pelle kinases (RLK) are a group of conserved signalling components that regulate developmental programs and responses to biotic and abiotic stresses. One of the largest RLK groups is formed by the Domain of Unknown Function 26 (DUF26) RLKs, also called Cysteine-rich Receptor-like Kinases (CRKs), which have been suggested to play important roles in the regulation of pathogen defence and programmed cell death. Despite the vast number of RLKs present in plants, however, only a few of them have been functionally characterized. Results: We examined the transcriptional regulation of all Arabidopsis CRKs by ozone (O 3), high light and pathogen/ elicitor treatment-conditions known to induce the production of reactive oxygen species (ROS) in various subcellular compartments. Several CRKs were transcriptionally induced by exposure to O 3 but not by light stress. O 3 induces an extracellular oxidative burst, whilst light stress leads to ROS production in chloroplasts. Analysis of publicly available microarray data revealed that the transcriptional responses of the CRKs to O 3 were very similar to responses to microbes or pathogen-associated molecular patterns (PAMPs). Several mutants altered in hormone biosynthesis or signalling showed changes in basal and O 3-induced transcriptional responses. Conclusions: Combining expression analysis from multiple treatments with mutants altered in hormone biosynthesis or signalling suggest a model in which O 3 and salicylic acid (SA) activate separate signaling pathways that exhibit negative crosstalk. Although O 3 is classified as an abiotic stress to plants, transcriptional profiling of CRKs showed strong similarities between the O 3 and biotic stress responses. Background Receptor-like/Pelle kinases (RLKs) are important components in the regulation of plant development, hormone signalling, abiotic, and biotic stress responses in plants. RLKs are serine-threonine protein kinases that typically contain a signal peptide, a variable extracellular domain, a transmembrane region, and a conserved intracellular protein kinase domain. The extracellular ligand-binding domain perceives signals and is commonly used to classify RLKs into distinct subgroups [1]. The RLKs are one of the largest gene families in Arabidopsis with more than