Enhanced Cytokine Synthesis of Leukocytes by a β‐Glucan Preparation, SCG, Extracted from a Medicinal Mushroom, Sparassis crispa
Enhanced Cytokine Synthesis of Leukocytes by a β‐Glucan Preparation, SCG, Extracted from a Medicinal Mushroom, Sparassis crispa
复制标题
通过从药用蘑菇、绣球菌中提取的 β-葡聚糖制剂 (SCG) 增强白细胞的细胞因子合成
DOI：
--
复制
发表时间：
2003
影响因子：
3.3
通讯作者：
N. Ohno
中科院分区：
文献类型：
--
作者：
S. Nameda;Toshie Harada;N. Miura;Y. Adachi;T. Yadomae;M. Nakajima;N. Ohno
松萝是日本近年来栽培的一种食用菌。它含有显著高含量（约40%）的6-支链1，3-β-D-葡聚糖，在小鼠中显示出抗肿瘤活性。我们最近纯化了一种称为“SCG”的β-葡聚糖制剂。因此，我们认为用人体志愿者的全血在体外检测SCG是值得的。本研究的重点是在体外人体系统中SCG的细胞因子生产力。观察到以下结果：（i）SCG剂量依赖性地增强人外周血的全血细胞培养物的IL-8合成。(ii)IL-8合成在PBMC和PMN培养物中均增强。(iii)在具有自体血浆的培养物中诱导IL-8合成，但在56°C处理后显著降低。(iv)热灭活胎牛血清（FCS）中的活性也较弱。(v)SCG依赖于剂量和动力学释放补体片段C5 a。(vi)在人血浆中检测到抗SCG天然抗体。从这些事实中，观察到SCG具有激活人白细胞和相关免疫系统的能力。
Abstract Sparassis crispa is edible mushroom recently cultivable in Japan. It contains significantly high content (∼40%) of 6‐branched 1,3‐β‐D‐glucan showing antitumor activity in mice. We recently purified a β‐glucan preparation designated as “SCG.” It was considered worth while to test SCG in vitro with whole blood collected from human volunteers. The present study is focusing on the cytokine productivity of SCG in an in vitro human system. The following results were observed: (i) SCG dose dependently enhanced IL‐8 synthesis of whole blood cell culture of human peripheral blood. (ii) IL‐8 synthesis was enhanced in both PBMC and PMN cultures. (iii) IL‐8 synthesis was induced in the culture with autologous plasma, but significantly reduced after 56°C treatment. (iv) The activity was also weak in heat inactivated fetal calf serum (FCS). (v) A complement fragment, C5a, was released by SCG dependently upon dose and kinetics. (vi) Anti‐SCG natural antibody was detected in human plasma. From these facts, SCG was observed to have the capacity to activate human leukocytes and related immune system.