Frequent reassortments may explain the genetic heterogeneity of rotaviruses: Analysis of Finnish rotavirus strains
Frequent reassortments may explain the genetic heterogeneity of rotaviruses: Analysis of Finnish rotavirus strains
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DOI：
10.1128/jvi.76.23.11793-11800.2002
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发表时间：
2002-12-01
影响因子：
5.4
通讯作者：
von Bonsdorff, CH
中科院分区：
文献类型：
--
作者：
Maunula, L;von Bonsdorff, CH
建立了芬兰 17 个流行季节（1980 年至 1997 年）期间的主要轮状病毒电泳型（e 型），并通过核苷酸测序和系统发育分析对代表性病毒分离株进行了研究。病毒分离株为 P[8]G1 型或 P[8]G4 型。 G1和G4菌株分别形成一个G1谱系(VP7-G1-1)和一个G4谱系。否则，它们属于两个与其 G 类型无关的 P[8] 谱系（VP4-P[8]1- 和 -2）。对从菌株中获得的所有 11 个 RNA 片段的部分序列进行的系统发育分析也揭示了除了定义 P 和 G 类型的基因片段之外的基因片段之间的遗传多样性。除了片段1、3和10之外，其他片段的序列可以分配给2至4个不同的遗传簇。这项研究的结果表明，除了编码 VP4 和 VP7 的 RNA 片段外，其他 RNA 片段也可能独立分离。总的来说，当确定了 11 个基因的系统发育簇时，9 个主要 e 型代表了 7 种不同的 RNA 片段组合。轮状病毒广泛的遗传多样性和 e 型数量可以通过频繁的遗传重排得到最好的解释。
The predominant rotavirus electropherotypes (e-types) during 17 epidemic seasons (1980 through 1997) in Finland were established, and representative virus isolates were studied by nucleotide sequencing and phylogenetic analysis. The virus isolates were either P[8]G1 or P[8]G4 types. The G1 and G4 strains formed one G1 lineage (VP7-G1-1) and one G4 lineage, respectively. Otherwise, they belonged to two P[8] lineages (VP4-P[8]1- and -2) unrelated to their G types. Phylogenetic analysis of partial sequences of all 11 RNA segments obtained from the strains also revealed genetic diversity among gene segments other than those defining P and G types. With the exception of segments 1, 3, and 10, the sequences of the other segments could be assigned to 2 to 4 different genetic clusters. The results of this study suggest that, in addition to the RNA segments encoding VP4 and VP7, the other RNA segments may segregate independently as well. In total, the 9 predominant e-types represented 7 different RNA segment combinations when the phylogenetic clusters of their 11 genes were determined. The extensive genetic diversity and number of e-types among rotaviruses are best explained by frequent genetic reassortment.