The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland
The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland
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DOI：
10.1046/j.1439-0450.2003.00628.x
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发表时间：
2003-04-01
期刊：
影响因子：
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通讯作者：
Cullor, JS
中科院分区：
文献类型：
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作者：
Alluwaimi, AM;Leutenegger, CM;Cullor, JS
采用TaqMan(R)实时定量聚合酶链式反应技术，研究了金黄色葡萄球菌感染牛乳腺全乳细胞中IL-2、IL-6、IL-12p40、干扰素-γ、肿瘤坏死因子-α和粒细胞-单核细胞集落刺激因子的转录活性。分别于感染后7、24、32h检测细胞因子转录活性。IL-12和TNF-α水平在PI后24 h显著升高，32 h后急剧下降。32hpi时IL-2水平下降。IL-12和干扰素-γ在24 h pi时有显著的交互作用。IL-12和TNF-α转录水平的显著升高很可能表明它们在金黄色葡萄球菌感染时对牛乳腺免疫反应的调节中起重要作用。IL-2的抑制可反映金黄色葡萄球菌乳房炎的抑制性。
TaqMan(R) real time PCR was used to study the transcriptional activity of the bovine IL-2, IL-6, IL-12p40, IFN-gamma , TNF-alpha and granulocyte-monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus . Cytokine transcriptional activity was monitored at 7, 24 and 32 h Post-infection (Pi). IL-12 and TNF-alpha levels were significantly elevated at 24 h Pi followed by sharp decrease at 32 h pi. IL-2 level was decreased at 32 h pi. IL-12 and IFN-gamma showed a significant interaction at 24 h pi. The significant elevations of the IL-12 and TNF-alpha transcriptional level most likely indicate their important role in regulation of the immune responses of bovine mammary gland in S. aureus infection. Depression of IL-2 could reflect the suppressive nature of the S. aureus mastitis.