Soy Protein Isolate Modified Metabolic Phenotype and Hepatic Wnt Signaling in Obese Zucker Rats
Soy Protein Isolate Modified Metabolic Phenotype and Hepatic Wnt Signaling in Obese Zucker Rats
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DOI：
10.1055/s-0031-1287855
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发表时间：
2011-10-01
影响因子：
2.2
通讯作者：
Davis, J. E.
中科院分区：
文献类型：
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作者：
Cain, J.;Banz, W. J.;Davis, J. E.
我们之前已经证明含有完整植物雌激素的大豆分离蛋白（SPI）可以预防肥胖相关的功能障碍。最近的数据表明，大豆成分可能作为脂肪组织（AT）和肝脏中脂肪生成程序的调节器。因此，目前的研究是为了确定SPI的有益作用是否与脂肪生成调节因子的变化有关，如Wnt信号级联。为此，瘦肉大鼠（LZR）和肥胖大鼠（OZR）被给予含有SPI、酪蛋白酸钠或乳清蛋白的等热量和等氮饲料17周。在终止时，SPI增加了啮齿动物的体重和总脂肪，这与脂肪细胞大小和数量的增加相对应。此外，提供SPI的OZR大鼠炎症、高胆固醇血症和肝脂肪变性指标均降低。SPI明显改变了肝脏中几种典型和非典型Wnt信号中间体的转录丰度，而不是AT。总的来说，这些数据证实了保护性SPI可能通过调节脂肪生成程序来减轻肥胖相关的代谢功能障碍，正如AT形态和肝脏Wnt信号的变化所证明的那样。总的来说，本研究证实了大豆蛋白及其生物活性成分在预防和治疗肥胖相关合并症方面的潜在应用。
We have previously shown that soy protein isolate (SPI) with intact phytoestrogen content prevented obesity-related dysfunction. Recent data have suggested that soy ingredients may act as regulators of adipogenic programming in adipose tissue (AT) and liver. Thus, the current study was undertaken to determine whether the beneficial effects of SPI are linked to changes in adipogenic regulators, such as the Wnt signaling cascade. For this, lean (LZR) and obese Zucker (OZR) rats were provided isocaloric and isonitrogenous diets containing SPI, sodium caseinate, or dairy whey protein for 17 weeks. At termination, SPI increased body weight and total adiposity in rodents, which corresponded with an increase in both adipocyte size and number. Furthermore, markers of inflammation, hypercholesterolemia, and hepatic steatosis were all reduced in OZR rats provided SPI. Transcript abundance of several canonical and noncanonical Wnt signaling intermediates in liver, but not AT, was distinctly modified by SPI. Collectively, these data confirm the protective SPI attenuated obesity-related metabolic dysfunction conceivably through regulation of adipogenic programming, as evident by changes in AT morphology and hepatic Wnt signaling. Collectively, this study confirmed the potential utilization of soy protein and its bioactive ingredients for prevention and treatment of obesity-related comorbidities.