ASTROCYTE ASSOCIATIONS WITH NODES OF RANVIER - ULTRASTRUCTURAL ANALYSIS OF HRP-FILLED ASTROCYTES IN THE MOUSE OPTIC-NERVE
ASTROCYTE ASSOCIATIONS WITH NODES OF RANVIER - ULTRASTRUCTURAL ANALYSIS OF HRP-FILLED ASTROCYTES IN THE MOUSE OPTIC-NERVE
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DOI：
10.1007/bf01184072
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发表时间：
1994-08-01
期刊：
影响因子：
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通讯作者：
BERRY, M
中科院分区：
文献类型：
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作者：
BUTT, AM;DUNCAN, A;BERRY, M
星形胶质细胞参与朗氏结的功能，因为它们的结周突起与节点处的轴突膜形成接触。我们在完整的小鼠视神经中用辣根过氧化物酶离子电渗填充星形胶质细胞，以解决结周突起和星形胶质细胞三维结构之间的精确关系。我们确认，节点接触形成的单个过程，几乎完全包围节点，或微妙的，手指状的预测，从较大的过程，使离散的节点接触。单个结周突起可以与一个结形成多个接触，并且结与来自多个星形胶质细胞的突起接触。结周突起起源于较大的突起，这些突起终止于血管和软脑膜上的终足，以及随后终止于结的侧支;后者可能专门为结服务。结周接触也直接由细胞体的索马和胞质扩张形成。主突和侧枝与节点形成多个联系，这些联系往往成簇出现。因此，所有的星形胶质细胞与淋巴结、血管和软膜下胶质界膜形成了多个接触。我们的结论是，在小鼠视神经的一个专门的星形胶质细胞没有形成的结周过程。
Astrocytes are implicated in the function of nodes of Ranvier because their perinodal processes form contacts with the axonal membrane at nodes. We have filled astrocytes iontophoretically with horseradish peroxidase in the intact mouse optic nerve to resolve the precise relationship between perinodal processes and astrocyte three dimensional structure. We confirm that nodal contacts were formed either by single processes which almost completely enveloped nodes, or by delicate, finger-like projections from larger processes which made discrete nodal contacts. A single perinodal process can form multiple contacts with a node and nodes were contacted by processes from more than one astrocyte. Perinodal processes emanated from larger processes, which terminated as end-feet on blood vessels and at the pia, as well as collateral branches which subsequently ended at nodes; these latter may specifically subserve nodes. Perinodal contacts were also formed directly by the soma and cytoplasmic expansions of the cell body. Both primary processes and collateral branches formed multiple associations with nodes which often appeared in clusters. Thus, all astrocytes formed multiple contacts with nodes, blood vessels and the subpial glia limitans. We conclude that perinodal processes are not formed by a specialized astrocyte in the mouse optic nerve.