AUXOTROPHIC VARIANTS OF PSEUDOMONAS-AERUGINOSA ARE SELECTED FROM PROTOTROPHIC WILD-TYPE STRAINS IN RESPIRATORY-INFECTIONS IN PATIENTS WITH CYSTIC-FIBROSIS
AUXOTROPHIC VARIANTS OF PSEUDOMONAS-AERUGINOSA ARE SELECTED FROM PROTOTROPHIC WILD-TYPE STRAINS IN RESPIRATORY-INFECTIONS IN PATIENTS WITH CYSTIC-FIBROSIS
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DOI：
10.1128/jcm.33.1.37-40.1995
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发表时间：
1995-01-01
影响因子：
9.4
通讯作者：
PITT, TL
中科院分区：
文献类型：
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作者：
BARTH, AL;PITT, TL
从20例囊性纤维化（CF）患者中分离出24株营养依赖型（营养缺陷型）铜绿假单胞菌，测定其氨基酸需要量。甲硫氨酸是最常见的所需的单一氨基酸（24个分离株中的9个），其次是亮氨酸和精氨酸或鸟氨酸。总共证明需要12种不同的化合物或化合物组合。通过脉冲场凝胶电泳中DNA的宏观限制性分析，比较了来自同一患者的嗜热和原养型菌株对。18对分析中的13个在用XbaI消化DNA后呈现相同的限制性片段长度多态性谱。剩下的三对显示出77%，91%和98%的相似性百分比，并且由于其DNA的过度降解，两对的概况无法进行比较。这些结果表明，定殖于同一CF患者的营养缺陷型和原养型铜绿假单胞菌分离物构成了同基因组，并提高了在CF患者的肺部感染过程中从原养型群体中选择营养缺陷型的可能性，
Twenty-four nutritionally dependent (auxotrophic) Pseudomonas aeruginosa strains were isolated from 20 cystic fibrosis (CF) patients and tested for their amino acid requirements, Two different methods were necessary to identify the nutritional status of all isolates. Methionine was the most common single amino acid required (9 of 24 isolates), followed by leucine and arginine or ornithine. Ln total, a requirement for 12 different compounds or combination of compounds was demonstrated. Auxotrophic and prototrophic pairs of isolates from the same patient were compared by macrorestriction analysis of DNA in pulsed-field gel electrophoresis. Thirteen of 18 pairs analyzed presented identical restriction fragment length polymorphism profiles following digestion of DNA with XbaI. Three of the remaining pairs showed percentage similarities of 77, 91, and 98%, and the profiles of two pairs could not be compared because of the excessive degradation of their DNA. These results suggest that auxotrophic and prototrophic P. aeruginosa isolates colonizing the same CF patient constitute an isogenic group and raise the possibility that auxotrophs are selected from the prototrophic population during the course of pulmonary infection in CF patients,