An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material
An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material
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DOI：
10.1002/jbm.b.10050
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发表时间：
2003-10-15
影响因子：
3.4
通讯作者：
Smith, D
中科院分区：
文献类型：
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作者：
Mukherjee, DP;Tunkle, AS;Smith, D
本研究的目的是开发一种糊状的合成骨移植物。这里报道的是在大鼠临界尺寸缺损模型中使用的壳聚糖谷氨酸(Protosan(R))和羟基磷灰石糊剂(简称糊剂)的评估结果。按照动物审查委员会批准的方案，在大鼠颅骨上制造直径八毫米的颅骨缺损。研究了五组：（1）空对照，（2）仅用糊剂填充缺损，（3）用含有骨髓抽吸物的糊剂填充缺损，（4）用含有BMP-2的糊剂填充缺损，以及（5）用含有从骨髓抽吸物培养的成骨细胞的糊剂填充缺损。处死间隔为9周和18周。收获含有缺损的颅骨，并通过双能X射线骨密度测定法测定骨矿物质密度(BMD)。还进行了推出强度测量。空对照组在第9周和第18周时的BMD值均显着低于其他组。样品之间的机械性能，即推出强度以及推出负载和位移下的面积没有显着差异。戈德纳三色染色未脱钙切片的组织学检查显示，缺损区域存在矿化骨针，这些骨针在充满糊剂和骨髓抽吸物培养的成骨细胞的区域更为突出。因此，本研究表明，从骨髓抽吸物中培养的壳聚糖谷氨酸和含羟基磷灰石成骨细胞的糊剂将是修复骨缺损的有效材料。 (C) 2003 年 Wiley 期刊公司。
The objective of this study was to develop a synthetic bone graft in a paste form. Reported here are the results of the evaluation of a paste of chitosan glutamate (Protosan(R)) and hydroxyapatite (referred to as a paste) used in a critical size defect model in rats. Eight-millimeter-diameter cranial defects were made in rat calvaria following a protocol approved by the animal review committee. Five groups were studied: (1) empty control, (2) defect filled with paste only, (3) defect filled with the paste containing bone-marrow aspirate, (4) defect filled with paste containing BMP-2, and (5) defect filled with paste containing osteoblasts cultured from bone-marrow aspirate. The sacrifice intervals were 9 and 18 weeks. Calvaria containing the defect were harvested, and the bone mineral density (BMD) was determined by dual energy X-ray absorptiometry. Push-out strength measurements were also performed. The BMD values of empty control were significantly lower than those of other groups at both 9 and 18 weeks. The mechanical properties, that is, push-out strengths and area under the push-out load and displacement were not significantly different between the samples. Histological examination of Goldner-trichromestained undecalcified sections showed the presence of mineralized bone spicules in the defect areas that were more prominent in those filled with paste and osteoblasts cultured from bone-marrow aspirate. Hence, this study demonstrated that the paste of chitosan glutamate and hydroxyapatite-containing osteoblasts cultured from bone-marrow aspirate would be an effective material to repair bone defects. (C) 2003 Wiley Periodicals, Inc.