The importance of platelets for the isolated lamb heart perfused at 13°C☆
The importance of platelets for the isolated lamb heart perfused at 13°C☆
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血小板对于13°C离体羔羊心脏灌注的重要性☆
DOI：
10.1016/0022-4804(79)90058-1
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发表时间：
1979
影响因子：
2.2
通讯作者：
A. Erdmann
中科院分区：
文献类型：
--
作者：
J. Suaudeau;A. Carvalho;W. Austen;A. Erdmann
为了研究在血浆灌注液中加入功能性血小板是否可以改善在13°C下保存18小时的离体羔羊心脏，用贫血小板血浆灌注13个心脏。他们表现出冠状动脉血管阻力增加，水肿，并在最终复温后左心室评估功能差。另外12个心脏用从流通式离心机收集的富血小板血浆（PRP）灌注。血小板在循环中聚集并改变了体外功能。这些心脏也变得水肿，在最终评估时不能耐受左心房灌注。Pluronic F68（一种非离子表面活性剂）加入PRP可防止灌注回路中的血小板聚集，并保护灌注液中的血小板功能。用这种改良PRP灌注的6个心脏具有稳定的冠状血管阻力，没有显著的体重增加，并且在终末复温时剧烈收缩，6个中有4个能够支持左心房灌注。这些结果表明，未聚集的，功能性血小板减少水肿和稳定冠状动脉血管阻力在离体心脏灌注在寒冷的温度。
To investigate whether or not the addition of functional platelets to a plasma perfusate could improve the preservation of isolated lamb hearts at 13°C for 18 hr, 13 hearts were perfused with platelet-poor plasma. They exhibited a rise in coronary vascular resistance, edema, and poor function upon terminal rewarming for left ventricular assessment. Twelve other hearts were perfused with platelet-rich plasma (PRP) harvested from a flow-through centrifuge. Platelets aggregated in the circuit and had alteredin vitrofunction. These hearts also became edematous and could not tolerate left atrial perfusion at final assessment. The addition of Pluronic F68, a nonionic surface active agent, to PRP prevented platelet aggregation in the perfusion circuit and preserved platelet function in the perfusate. Six hearts perfused with this modified PRP had stable coronary vascular resistance, had no significant weight gain, and contracted vigorously upon terminal rewarming, with 4 out of 6 able to support left atrial perfusion. These results suggest that unaggregated, functional platelets reduce edema and stabilize coronary vascular resistance in the isolated heart perfused at cold temperatures.