Incorporating platelet-rich plasma into coaxial electrospun nanofibers for bone tissue engineering
Incorporating platelet-rich plasma into coaxial electrospun nanofibers for bone tissue engineering
复制标题
将富含血小板的血浆纳入同轴电纺纳米纤维中，用于骨组织工程。
DOI：
10.1016/j.ijpharm.2018.06.020
复制
发表时间：
2018-08-25
影响因子：
5.8
通讯作者：
Li, Zubing
中科院分区：
文献类型：
--
作者：
Cheng, Gu;Ma, Xiao;Li, Zubing
富血小板血浆(PRP)被用于骨组织修复的治疗，因为浓缩的血小板可以释放大量的成骨相关生长因子。然而，它的临床应用是有限的，因为从PRP暂时释放的生长因子被迅速降解。本研究旨在通过同轴静电纺丝将PRP衍生的生长因子引入SF/PCL/PVA纳米纤维中，以确定生长因子的释放情况以及这些生长因子的存在如何增强纳米纤维的成骨能力。制备了含有不同比例的PRP和PVA的支架，并对其进行了表征。然后，我们量化了随着时间的推移纳米纤维中生长因子的释放，并评估了MSCs的增殖、迁移和成骨分化。通过将PRP/MSCs/CS/β-TCP复合载体植入裸鼠背部皮肤和修复C57BL/6小鼠颅骨缺损模型，评价了含有PRP的核壳复合体的体内成骨能力。采用免疫荧光染色、MU CT及组织学观察等方法对治疗结果进行分析。结果表明，当PRP-5%PVA溶液比例为7：1时，同轴纳米纤维中PRP含量较高，纤维直径分布更均匀。由于骨髓间充质干细胞(BMSCs)的增殖和迁移增加，支架的生物活性增强。当细胞接种并培养在负载PRP的纳米纤维垫上时，II型胶原的表达也增加。此外，PRP-NFS8周后也促进了新骨的形成。综上所述，本研究表明PRP的掺入对同轴纳米纤维垫的生物活性和成骨能力有积极的影响。这种纳米纤维垫可能在骨组织工程的各种应用中被证明是有益的。
Platelet-rich plasma (PRP) is used in therapy for bone tissue repair because an abundance of osteogenesis-related growth factors can be released from the concentrated platelets. However, its clinical use is limited because growth factors, temporally released from PRP, are degraded rapidly. This study aimed to incorporate PRP-derived growth factors into SF/PCL/PVA nanofibers by coaxial electrospinning to determine the release profiles of growth factors and how the presence of these growth factors enhances the osteogenic abilities of the nanofibers. Scaffolds containing different ratios of PRP and PVA were prepared and characterized. We then quantified the release of growth factors from the nanofibers over time, and evaluated the proliferation, migration and osteogenic differentiation of MSCs. The in vivo osteogenic capacity of the PRP-containing core-shell NFS was also evaluated by transplanting the PRP/MSCs/CS/beta-TCP compounds into the skin on the back of nude mice and by treating cranial defects of C57BL/6 mice. The results of such treatments were analyzed by immunofluorescent staining, mu computed tomography (mu CT), and histological observation. The results show that coaxial nanofibers with a PRP-5% PVA solution ratio of 7:1 contained a relatively high amount of PRP and exhibited a more uniform distribution of fiber diameters. The bioactivity of the scaffolds was enhanced due to the increased proliferation and migration of bone marrow mesenchymal stem cells (BMSCs). When the cells were inoculated and cultured on the PRP-loaded nanofibrous mats, the expression of collagen type II also increased. Furthermore, new bone formation was also promoted by PRP-NFS after 8 weeks of implantation. In conclusion, this study shows that the incorporation of PRP had positive effects on the bioactivity and osteogenic ability of coaxial nanofibrous mats. Such nanofibrous mats may prove beneficial in various applications of bone tissue engineering.