Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter.
Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter.
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DOI：
10.1111/mmi.12834
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发表时间：
2015-04
影响因子：
3.6
通讯作者：
Biteen JS
中科院分区：
文献类型：
--
作者：
Haas BL;Matson JS;DiRita VJ;Biteen JS
Vibrio cholerae causes the human disease cholera by producing a potent toxin. The V. cholerae virulence pathway involves an unusual transcription step: the bitopic inner-membrane proteins TcpP and ToxR activate toxT transcription. As ToxT is the primary direct transcription activator in V. cholerae pathogenicity, its regulation by membrane-localized activators is key in the disease process. However, the molecular mechanisms by which membrane-localized activators engage the transcription process have yet to be uncovered in live cells. Here we report the use of super-resolution microscopy, single-molecule tracking, and gene knockouts to examine the dynamics of individual TcpP proteins in live V. cholerae cells with <40-nm spatial resolution on a 50-ms timescale. Single-molecule trajectory analysis reveals that TcpP diffusion is heterogeneous and can be described by three populations of TcpP motion: one fast, one slow, and one immobile. By comparing TcpP diffusion in wild type V. cholerae to that in mutant strains lacking either toxR or the toxT promoter, we determine that TcpP mobility is greater in the presence of its interaction partners than in their absence. Our findings support a mechanism in which ToxR recruits TcpP to the toxT promoter for transcription activation.
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影响因子：
3.2
作者：
KOLIBACHUK, D;GREENBERG, EP
通讯作者：
GREENBERG, EP
影响因子：
3.6
作者：
Lassak, Kerstin;Peeters, Eveline;Albers, Sonja-Verena
通讯作者：
Albers, Sonja-Verena
影响因子：
48
作者：
Biteen, Julie S.;Thompson, Michael A.;Tselentis, Nicole K.;Bowman, Grant R.;Shapiro, Lucy;Moerner, W. E.
通讯作者：
Moerner, W. E.
DOI：
10.3390/molecules190812116
发表时间：
2014-08-13
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Haas BL;Matson JS;DiRita VJ;Biteen JS
通讯作者：
Biteen JS
DOI：
10.1126/science.1222981
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berk V;Fong JC;Dempsey GT;Develioglu ON;Zhuang X;Liphardt J;Yildiz FH;Chu S
通讯作者：
Chu S