Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling
Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling
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DOI：
10.1007/s11103-004-5920-2
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发表时间：
2004-12-01
影响因子：
5.1
通讯作者：
Komatsu, S
中科院分区：
文献类型：
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作者：
Konishi, H;Yamane, H;Komatsu, S
二磷酸果糖醛缩酶是一种糖酵解酶，在赤霉素（GA）处理的水稻根中其活性增加。为了研究醛缩酶与根系生长的关系，对赤霉素诱导的根系醛缩酶进行了表征。当0.1 μ M GA（3）外源添加到水稻根中时，GA（3）促进了醛缩酶积累的增加。醛缩酶在根中积累丰富，特别是在顶端区域。为了研究醛缩酶功能对根生长的影响，构建了表达反义醛缩酶的转基因水稻植株。与载体对照相比，转醛缩酶反义基因水稻根系生长受到抑制。虽然用0.1 μ M GA（3）处理的载体对照水稻根中的醛缩酶活性增加了25%，但在醛缩酶反义转基因水稻根中，0.1 μ M GA（3）处理的FBPA活性增加很少。此外，在水稻根中，醛缩酶与针对液泡H+-ATPase的抗体共免疫沉淀。在OsCDPK 13反义转基因水稻的根中，即使在GA处理后，醛缩酶也没有积累（3）。这些结果表明，糖酵解途径功能的激活促进了根的生长，GA（3）诱导的根醛缩酶可能通过OsCDPK 13调节。醛缩酶与根中的液泡H +-ATP酶物理结合，并可调节液泡H+-ATP酶介导的决定根长度的细胞伸长控制。
Fructose-bisphosphate aldolase is a glycolytic enzyme whose activity increases in rice roots treated with gibberellin (GA). To investigate the relationship between aldolase and root growth, GA-induced root aldolase was characterized. GA(3) promoted an increase in aldolase accumulation when 0.1 mu M GA(3) was added exogenously to rice roots. Aldolase accumulated abundantly in roots, especially in the apical region. To examine the effect of aldolase function on root growth, transgenic rice plants expressing antisense aldolase were constructed. Root growth of aldolase-antisense transgenic rice was repressed compared with that of the vector control transgenic rice. Although aldolase activity increased by 25% in vector control rice roots treated with 0.1 mu M GA(3), FBPA activity increased very little by 0.1 mu M GA(3) treatment in the root of aldolase-antisense transgenic rice. Furthermore, aldolase co-immunoprecipitated with antibodies against vacuolar H+-ATPase in rice roots. In the root of OsCDPK13-antisense transgenic rice, aldolase did not accumulate even after treatment with GA(3). These results suggest that the activation of glycolytic pathway function accelerates root growth and that GA(3)-induced root aldolase may be modulated through OsCDPK13. Aldolase physically associates with vacuolar H-ATPase in roots and may regulate the vacuolar H+-ATPase mediated control of cell elongation that determines root length.