Identification of an anellovirus and genomoviruses in ixodid ticks
Identification of an anellovirus and genomoviruses in ixodid ticks
复制标题
蜱蜱中指环病毒和基因病毒的鉴定
DOI：
10.1007/s11262-017-1520-5
复制
发表时间：
2017
期刊：
影响因子：
1.6
通讯作者：
A. Varsani
中科院分区：
文献类型：
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作者：
Kara Waits;M. Edwards;Ilaria N. Cobb;R. Fontenele;A. Varsani
蜱是吸血的蜘蛛纲动物，是几种重要的人类和动物病原体的载体。人们对与这些体外寄生虫相关的单链DNA（ssDNA）病毒知之甚少。作为鉴定蜱中存在的ssDNA病毒的初步研究的一部分，在宾夕法尼亚州东部（美国）收集了雌性美国犬蜱（Dermacentor variabilis）和黑腿蜱（Ixodes scapularis），并使用病毒宏基因组学方法分析了提取的病毒DNA。从D. variabilis（n = 2）和I.肩突病毒（n = 1）：两个属于Gemycirarvirus属，与属内其他成员共享< 63%的成对同一性;第三个属于Gemykolovirus属，与其他Gemykolovirus共享< 70%的成对同一性。此外，还从D. variabilis合并样本。
Ticks are blood-feeding arachnids that are vectors of several important human and animal pathogens. Little is known about single-stranded DNA (ssDNA) viruses that are associated with these ectoparasites. As part of a pilot study to identify ssDNA viruses present in ticks, female American dog ticks (Dermacentor variabilis) and blacklegged ticks (Ixodes scapularis) were collected in eastern Pennsylvania (USA), and the extracted viral DNA was analyzed using viral metagenomics approaches. Three genomoviruses were recovered from pooled samples of D. variabilis (n = 2) and I. scapularis (n = 1): two belonging to the genus Gemycircularvirus, sharing < 63% pairwise identity with other members within the genus; and the third belonging to the genus Gemykolovirus, sharing < 70% pairwise identity to other gemykoloviruses. Furthermore, a genome of an anellovirus belonging to the sharing 62–65% nucleotide identity with torque teno canis viruses (genus Thetatorquevirus) was also recovered from a D. variabilis pooled sample.