Novel whole blood transcriptome signatures of changes in maximal aerobic capacity in response to endurance exercise training in healthy women.
Novel whole blood transcriptome signatures of changes in maximal aerobic capacity in response to endurance exercise training in healthy women.
复制标题
健康女性耐力运动训练最大有氧能力变化的新型全血转录组特征。
DOI：
10.1152/physiolgenomics.00017.2023
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发表时间：
2023
影响因子：
4.6
通讯作者：
Seals,DouglasR
中科院分区：
文献类型：
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作者：
LaRocca,ThomasJ;Smith,MeghanE;Freeberg,KaitlinA;Craighead,DanielH;Helmuth,Timothy;Robinson,MatthewM;Nair,KSreekumaran;Bryan,AngelaD;Seals,DouglasR
最大有氧运动量[最大耗氧量(V̇o2max)]是发病率和死亡率的最强预测因子之一。有氧运动训练可提高V̇o2max，但个体间变异显著，生理原因不明。这种变异性背后的机制对延长人类健康寿命具有重大的临床意义。在这里，我们报告了一个新的转录组特征，与运动训练相关的ΔV̇o2max在全血中被检测到。我们使用核糖核酸序列来表征健康女性的ΔV̇o2max的转录特征，这些女性完成了一项为期16周的随机对照试验，比较了有监督的、高强度和低强度的有氧运动训练量和强度(4个训练组，完全交叉)。我们发现，在对有氧运动训练有强烈反应和很少有ΔV̇o2max的受试者中，基线基因表达存在显著差异，差异表达的基因/转录本主要与炎症信号和线粒体功能/蛋白质翻译有关。运动训练也以剂量依赖的方式调节了与健壮和少/无ΔV̇o2max相关的基线基因表达特征，并在该数据集和单独的数据集中预测了ΔV̇o2max。总体而言，我们的数据证明了使用全血转录组学来研究对相同运动训练刺激反应的个体间差异的生物学作用的潜在效用。
Maximal aerobic exercise capacity [maximal oxygen consumption (V̇o2max)] is one of the strongest predictors of morbidity and mortality. Aerobic exercise training can increase V̇o2max, but inter-individual variability is marked and unexplained physiologically. The mechanisms underlying this variability have major clinical implications for extending human healthspan. Here, we report a novel transcriptome signature related to ΔV̇o2maxwith exercise training detected in whole blood RNA. We used RNA-Seq to characterize transcriptomic signatures of ΔV̇o2maxin healthy women who completed a 16-wk randomized controlled trial comparing supervised, higher versus lower aerobic exercise training volume and intensity (4 training groups, fully crossed). We found significant baseline gene expression differences in subjects who responded to aerobic exercise training with robust versus little/no ΔV̇o2max, and differentially expressed genes/transcripts were mostly related to inflammatory signaling and mitochondrial function/protein translation. Baseline gene expression signatures associated with robust versus little/no ΔV̇o2maxwere also modulated by exercise training in a dose-dependent manner, and they predicted ΔV̇o2maxin this and a separate dataset. Collectively, our data demonstrate the potential utility of using whole blood transcriptomics to study the biology of inter-individual variability in responsiveness to the same exercise training stimulus.