Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity
Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity
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DOI：
10.1083/jcb.201910021
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发表时间：
2020-07-06
影响因子：
7.8
通讯作者：
Gladfelter, Amy S.
中科院分区：
文献类型：
--
作者：
Gerbich, Therese M.;McLaughlin, Grace A.;Gladfelter, Amy S.
生物分子凝聚是一种组织细胞溶质的方式，其中蛋白质和核酸共同组装成隔室。在多核丝状真菌棉阿舒囊菌中，RNA结合蛋白Whi3通过形成类似于缩合物的大分子结构来调节细胞周期和细胞极性。Whi3具有独特的空间定位和mRNA靶点，使其成为如何，何时以及在何处为缩合物建立特定身份的强大模型。我们鉴定了Whi3上在特定条件下差异磷酸化的残基，并产生了消除这种调节的突变体。这产生了分离的功能等位基因的细胞极性或核循环的功能，但不是两者兼而有之。这项研究表明，在生物分子凝聚物中，分子上单个残基的磷酸化可以提供特异性，从而在同一细胞中产生不同的功能特性。
Biomolecular condensation is a way of organizing cytosol in which proteins and nucleic acids coassemble into compartments. In the multinucleate filamentous fungus Ashbya gossypii, the RNA-binding protein Whi3 regulates the cell cycle and cell polarity through forming macromolecular structures that behave like condensates. Whi3 has distinct spatial localizations and mRNA targets, making it a powerful model for how, when, and where specific identities are established for condensates. We identified residues on Whi3 that are differentially phosphorylated under specific conditions and generated mutants that ablate this regulation. This yielded separation of function alleles that were functional for either cell polarity or nuclear cycling but not both. This study shows that phosphorylation of individual residues on molecules in biomolecular condensates can provide specificity that gives rise to distinct functional identities in the same cell.