Growth of mammalian cells at high oxygen concentrations.
Growth of mammalian cells at high oxygen concentrations.
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DOI：
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发表时间：
1989-09
影响因子：
4
通讯作者：
A. Oller;C. Buser;M. Tyo;W. Thilly
中科院分区：
文献类型：
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作者：
A. Oller;C. Buser;M. Tyo;W. Thilly
氧，虽然在体外和体内对哺乳动物细胞的生长是必需的，但已广泛报道在等于或高于用空气平衡的培养基中的氧浓度（约200 μ M）的浓度下是有毒的。因此，我们惊讶地注意到，二倍体人类B细胞系（TK 6）在暴露于380微M氧气时能够正常增殖。将该观察结果扩展至Vero（非洲绿色猴肾）细胞和产生抗体的Sp2/0衍生的鼠转染瘤。使用具有高氧转移能力的实验系统，我们确定了三种细胞系在80 μ M至910 μ M的受控氧浓度下的生长速率。这些细胞类型中的每一种都能够在高达360-380 μ M的氧浓度下正常生长。在氧浓度高于380 μ M时，观察到细胞的表观倍增时间显著增加。在60 μ M至410 μ M的浓度范围内，未观察到氧对TK 6细胞存活的不利影响。我们的结论是，在接近最大生长速率的指数生长观察到的Sp2/0衍生的细胞，TK 6，和Vero细胞在恒定的氧浓度高达400 μ M（两倍的空气饱和度）。这些发现对细胞和细胞衍生材料生产的过程控制具有重大意义。任何细胞培养容器的固有氧转移速率都可以通过提高氧气浓度而增加数倍。（250字处删节）
Oxygen, although essential to the growth of mammalian cells in vitro and in vivo, has been widely reported to be toxic at concentrations at or above the oxygen concentration in culture medium equilibrated with air (approximately 200 microM). We were therefore surprised to note that a diploid human B-cell line (TK6) was able to proliferate normally while exposed to 380 microM-oxygen. This observation was extended to Vero (African Green monkey kidney) cells, and Sp2/0-derived murine transfectomas producing antibody. Using an experimental system with a high capacity for oxygen transfer, we determined the growth rates of the three cell lines at controlled oxygen concentrations ranging from 80 microM to 910 microM. Each of these cell types was able to grow normally at oxygen concentrations up to 360-380 microM. At oxygen concentrations above 380 microM, a significant increase in the apparent doubling times of the cells was observed. No adverse effect of oxygen on TK6 cell survival was seen for concentrations ranging from 60 microM to 410 microM. We conclude that exponential growth at nearly maximum growth rates was observed for Sp2/0-derived cells, TK6, and Vero cells at constant oxygen concentrations up to 400 microM (twice air saturation). These findings have substantial implications for process control in the production of cells and cell-derived materials. The intrinsic oxygen transfer rate of any cell culture vessel can be increased severalfold by raising the oxygen gas concentration.(ABSTRACT TRUNCATED AT 250 WORDS)