Genome-Wide Comparative Chromosome Map between Human and the Forrest’s Pika (Ochotona forresti) Established by Cross-Species Chromosome Painting: Further Support for the Glires Hypothesis
Genome-Wide Comparative Chromosome Map between Human and the Forrest’s Pika (Ochotona forresti) Established by Cross-Species Chromosome Painting: Further Support for the Glires Hypothesis
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DOI：
10.1159/000317082
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发表时间：
2010-07
影响因子：
1.7
通讯作者：
J. Ye;W. Nie;J. Wang;W. Su;M. Jing;A. Graphodatsky;Fengtang Yang
中科院分区：
文献类型：
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作者：
J. Ye;W. Nie;J. Wang;W. Su;M. Jing;A. Graphodatsky;Fengtang Yang
鼠兔的比较基因组数据对于理解鼠形动物的核型进化具有重要意义。在这里，我们通过跨物种染色体绘制，首次建立了人类与Forrest鼠兔(Ochotona forrest i，2n=54)之间的全基因组染色体同源性。将我们的图谱与已发表的人与兔的比较染色体图谱相结合，建立了代表兔形目2个科：鼠兔科和兔科的鼠兔与家兔(Oryctolagus cunrulus，2n=44)之间的间接同源图谱。我们的结果表明：(1)大多数提出的祖先真丝虫联体或共线联会被保留在O.forrest i基因组中；(2)HSA1/10p联配是支持将兔形目和啮齿目划分为一个称为Glires的单系支系的两个标志性重排之一，也存在于O.forrest i基因组中；(3)Robertsonian易位导致了O.forrest i和O.cunrulus之间的核型差异。
Comparative genomic data for ochotonids (pikas) are important for understanding the karyotype evolution of lagomorphs. Here, we have established the first genome-wide chromosomal homologies between human and the Forrest’s pika (Ochotona forresti, 2n = 54) by cross-species chromosome painting. Integration of our map with the published comparative chromosome map between human and rabbit has enabled the establishment of an indirect homology map between Forrest’s pika and domestic rabbit (Oryctolagus cuniculus, 2n = 44), representing the 2 Lagomorpha families: Leporidae and Ochotonidae. Our results demonstrate that (1) most of the proposed ancestral eutherian syntenies or syntenic associations have been retained in the O. forresti genome; (2) the HSA1/10p association, one of the 2 signature rearrangements that were proposed to support the grouping of the orders Lagomorpha and Rodentia into a monophyletic clade called Glires, is also present in the O. forresti genome; and (3) Robertsonian translocations have contributed to the karyotype differences between O. forresti and O. cuniculus.