Membrane shape as a reporter for applied forces
Membrane shape as a reporter for applied forces
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DOI：
10.1073/pnas.0806814105
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
Heun‐Jin Lee;Eric L. Peterson;R. Phillips;W. Klug;Paul A. Wiggins
中科院分区：
文献类型：
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作者：
Heun‐Jin Lee;Eric L. Peterson;R. Phillips;W. Klug;Paul A. Wiggins
最近的进展使生物结构的三维重建在体内，从单一的蛋白质到多细胞结构的大小和复杂性。特别是，断层扫描和共聚焦显微镜已被利用来捕捉详细的三维构象的膜在细胞过程中，从病毒出芽和细胞器的维护吞噬作用。尽管有丰富的膜结构，但至今还没有通用的定量方法来解释它们。我们建议，通过模拟这些观察到的生物膜的形状流体脂质双层在机械平衡，外部施加的力，以及压力，张力和自发曲率可以直接从形状单独计算。为了说明这种技术的潜在力量，我们施加一个轴向力与光镊囊泡，并明确表明，所施加的力是等于从膜构象计算的力。
Recent advances have enabled 3-dimensional reconstructions of biological structures in vivo, ranging in size and complexity from single proteins to multicellular structures. In particular, tomography and confocal microscopy have been exploited to capture detailed 3-dimensional conformations of membranes in cellular processes ranging from viral budding and organelle maintenance to phagocytosis. Despite the wealth of membrane structures available, there is as yet no generic, quantitative method for their interpretation. We propose that by modeling these observed biomembrane shapes as fluid lipid bilayers in mechanical equilibrium, the externally applied forces as well as the pressure, tension, and spontaneous curvature can be computed directly from the shape alone. To illustrate the potential power of this technique, we apply an axial force with optical tweezers to vesicles and explicitly demonstrate that the applied force is equal to the force computed from the membrane conformation.