Chick production by in vitro fertilization of the fowl ovum.
Chick production by in vitro fertilization of the fowl ovum.
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通过家禽卵子的体外受精生产小鸡。
DOI：
10.1530/jrf.0.1000447
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
F. Hertelendy
中科院分区：
文献类型：
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作者：
K. Tanaka;T. Wada;O. Koga;Y. Nishio;F. Hertelendy
本研究的目的是通过体外受精并将受精卵移植到受体母鸡的输卵管中来生产活鸡。在总共76个移植的卵子中，53个卵子的蛋壳完全钙化，其中31个（58%）是可育的。尽管胚胎损失率很高，但12个受精卵暴露于0.05 ml（1:200稀释）的精液中，仍孵出了6只活鸡。10个对照卵子经人工授精后从输卵管中取出，随后移植给受体母鸡，孵出9只健康雏鸡。该实验方法为转基因雏鸡的生产提供了有益的模型。
The aim of this study was to produce viable chicks by in vitro fertilization and transfer of fertilized ova to the oviduct of recipient hens. Out of a total of 76 transferred ova, 53 were laid with fully calcified shells, 31 of which were fertile (58%). Despite the high rate of embryonic loss, six live chicks were hatched from 12 fertile ova exposed to 0.05 ml of semen (1:200 dilution). Nine healthy chicks were hatched from ten control ova which were recovered from the oviduct following artificial insemination and subsequent transfer to recipient hens. This experimental approach provides a useful model for production of transgenic chicks.