Simultaneous determination of 8-hydroxydeoxyguanosine, a marker of oxidative stress, and creatinine, a standardization compound, in urine
Simultaneous determination of 8-hydroxydeoxyguanosine, a marker of oxidative stress, and creatinine, a standardization compound, in urine
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DOI：
10.2486/indhealth.43.333
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发表时间：
2005-04-01
影响因子：
2
通讯作者：
Kawai, K
中科院分区：
文献类型：
--
作者：
Kasai, H;Svoboda, P;Kawai, K
最近，H. Kasai 报道了一种通过高效液相色谱与电化学检测器 (HPLC-ECD) 联用来自动、精确地分析尿液中 8-羟基脱氧鸟苷 (8-OH-dG) 的方法。它基于通过阴离子交换色谱法进行的净化步骤以及在 ECD 检测之前使用反相色谱法进行的进一步纯化步骤。在本次通讯中，我们报告了一种同时测定 8-OH-dG 和肌酐的方法，肌酐是使尿液中 8-OH-dG 排泄正常化的内标。
Recently, H. Kasai reported an automatic, precise method of 8-hydroxydeoxyguanosine (8-OH-dG) analysis in urine by high performance liquid chromatography coupled to an electrochemical detector (HPLC-ECD). It is based on a cleaning-up step by anion-exchange chromatography and a further purification step using reverse phase chromatography before detection by the ECD. In this communication, we report a method for the simultaneous determination of 8-OH-dG and creatinine, an internal standard for normalizing the excretion of 8-OH-dG in urine.