Porcine acellular dermal matrix accelerates wound healing through miR-124-3p.1 and miR-139-5p
Porcine acellular dermal matrix accelerates wound healing through miR-124-3p.1 and miR-139-5p
复制标题
猪脱细胞真皮基质通过 miR-124-3p.1 和 miR-139-5p 加速伤口愈合
DOI：
10.1016/j.jcyt.2020.04.042
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发表时间：
2020-09-01
期刊：
影响因子：
4.5
通讯作者：
Xie, Julin
中科院分区：
文献类型：
--
作者：
Chen, Xiaodong;Yang, Ronghua;Xie, Julin
背景目的：皮肤伤口处理是一个主要的健康问题，并在世界范围内造成巨大的经济负担。先前的研究表明，伤口愈合是一个高度协调的过程，包括上皮化、血管生成、重塑和瘢痕形成。这种进展需要干细胞的自我更新、保存和修复特性。然而，我们对损伤后的详细内部调节机制和加速伤口愈合的方法的了解有限。方法：我们前期研究发现，猪脱细胞真皮基质（ADM）通过表皮干细胞（ESCs）促进创面愈合和瘢痕形成，这一过程与体内miRNA水平的改变有关。在本研究中，我们研究了猪ADM处理对ESCs中mirna的调控作用。结果：我们报道了猪ADM处理降低了miR-124-3p的水平。1和miR-139-5p在伤口中的表达。mir - 124 - 3 - p。1和miR-139-5p分别通过直接靶向ESCs中的mirna抑制JAG1和Notch1的表达。结论：本研究表明，猪ADM诱导miR-124-3p下调。1/139-5p在创面中表达，JAG1/Notch1在ESCs中表达上调，从而促进皮肤创面愈合。(c) 2020年国际细胞和基因治疗学会。Elsevier Inc.出版。版权所有。
Background aims: Cutaneous wound management is a major health problem and imposes a huge economic burden worldwide. Previous studies have demonstrated that wound healing is a highly coordinated process including epithelialization, angiogenesis, remodeling and scarring. This progression requires self-renewal, preservation and repair properties of stem cells. However, our understanding of the detailed internal regulatory mechanism following injury and the means to accelerate wound healing are limited.Methods: Our previous research revealed that porcine acellular dermal matrix (ADM) effectively promotes wound healing and scar formation through epidermal stem cells (ESCs), and this process is relevant to the alteration of internal miRNA levels. In this study, we investigated the regulatory function of porcine ADM treatment on miRNAs in ESCs.Results: We report that the treatment of porcine ADM reduced the levels of miR-124-3p.1 and miR-139-5p in wounds. MiR-124-3p.1 and miR-139-5p inhibited the expression of JAG1 and Notch1, respectively, by directly targeting miRNAs in ESCs.Conclusions: This work demonstrates that porcine ADM induced down-regulation of miR-124-3p.1/139-5p in wounds and up-regulation of JAG1/Notch1 in ESCs, thus enhancing cutaneous wound healing. (c) 2020 International Society for Cell and Gene Therapy. Published by Elsevier Inc. All rights reserved.