C-MET MESSENGER-RNA OVEREXPRESSION IN HUMAN HEPATOCELLULAR-CARCINOMA
C-MET MESSENGER-RNA OVEREXPRESSION IN HUMAN HEPATOCELLULAR-CARCINOMA
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DOI：
10.1016/0270-9139(94)90057-4
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发表时间：
1994-01-01
期刊：
影响因子：
13.5
通讯作者：
BARTRONS, R
中科院分区：
文献类型：
--
作者：
BOIX, L;ROSA, JL;BARTRONS, R
本研究旨在评估c-met过度表达在人肝细胞癌中的存在，并确定此特征是否与明确的临床或病理特征相关。c-met的表达，确定了一个特定的探针（人Met原癌基因）在18个肿瘤和非肿瘤的肝脏样本中获得的18例肝细胞癌患者提交手术治疗的Northern-blot杂交。18例肝细胞癌中有8例表现出c-met过表达，与周围肝脏相比，通过光密度测定法检测，c-met过表达增加2倍至10倍。相比之下，其余10例c-met表达与周围非肿瘤肝组织几乎相同。c-met的过度表达与年龄、性别、病因学或潜在肝脏疾病的功能状态，或肿瘤的大小、分化程度或假包膜浸润的存在以及额外肿瘤性结节的存在无关。这些数据表明，几乎一半的人肝细胞癌表现出c-met过表达。然而，该特征的生物学相关性尚不清楚。
This study was aimed at assessing the presence of c-met overexpression in human hepatocellular carcinoma and at determining whether this feature is associated with a definite clinical or pathological characteristic. Expression of c-met was determined by Northern-blot hybridization of a specific probe (human met proto-oncogene) in 18 tumoral and nontumoral liver samples obtained in 18 cirrhotic patients with hepatocellular carcinoma submitted to surgical treatment. Eight of the 18 hepatocellular carcinomas exhibited c-met overexpression, with an increase ranging between 2-fold and 10-fold when compared by densitometry with the surrounding liver. By contrast, in the remaining 10 cases c-met expression was almost identical to that of the surrounding nontumoral liver tissue. Overexpression of c-met was not related to either the age, sex, etiology or functional status of the underlying liver disease, or to the size of the tumor, to its differentiation degree or to the presence of pseudocapsule invasion and existence of additional neoplastic nodules. These data indicate that almost half of the human hepatocellular carcinomas exhibit c-met overexpression. Nevertheless, the biological relevance of this characteristic is not known.