Intraspecific violation of genetic colinearity and its implications in maize
Intraspecific violation of genetic colinearity and its implications in maize
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DOI：
10.1073/pnas.132259199
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发表时间：
2002-07-09
影响因子：
11.1
通讯作者：
Dooner, HK
中科院分区：
文献类型：
--
作者：
Fu, HH;Dooner, HK
尽管等位基因序列可能存在很大差异，但一般认为，一个个体中的每个基因在同一物种的另一个个体中都会有一个等位基因对应物。我们在此报告，这一假设在玉米中并不成立。我们对两个不同玉米品系的bz基因组区域进行了超过100kb的测序，发现它们之间存在巨大差异。首先，反转录转座子簇（构成玉米中大部分重复DNA）在组成和相对于bz区域基因的位置上有显著差异。其次，也是更重要的是，两个品系之间的基因本身存在差异，这表明在同一物种内遗传微共线性可能被打破。我们的发现与玉米以及可能其他生物的杂种优势的潜在遗传基础以及遗传距离的测量有关。
Although allelic sequences can vary extensively, it is generally assumed that each gene in one individual will have an allelic counterpart in another individual of the same species. We report here that this assumption does not hold true in maize. We have sequenced over 100 kb from the bz genomic region of two different maize lines and have found dramatic differences between them. First, the retrotransposon clusters, which comprise most of the repetitive DNA in maize, differ markedly in make-up and location relative to the genes in the bz region. Second, and more importantly, the genes themselves differ between the two lines, demonstrating that genetic microcolinearity can be violated within the same species. Our finding has bearing on the underlying genetic basis of hybrid vigor in maize, and possibly other organisms, and on the measurement of genetic distances.