CD8(+) T cell activation by murine erythroblasts infected with malaria parasites.
CD8(+) T cell activation by murine erythroblasts infected with malaria parasites.
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DOI：
10.1038/srep01572
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发表时间：
2013
影响因子：
4.6
通讯作者：
Hisaeda H
中科院分区：
文献类型：
--
作者：
Imai T;Ishida H;Suzue K;Hirai M;Taniguchi T;Okada H;Suzuki T;Shimokawa C;Hisaeda H
最近的研究表明，一些人类疟疾寄生虫种恶性疟原虫和间日疟原虫寄生于成红细胞;然而，这一点的生物学和临床意义尚不清楚。为了进一步研究，我们产生了表达GFP-卵清蛋白（OVA）的啮齿动物疟疾寄生虫（约氏疟原虫17 XNL）。证实了它对成红细胞的感染性，并且寄生的成红细胞能够引发疟疾感染。实验表明，MHC I类分子在被寄生的成红细胞上高度表达。由于CD 8 + T细胞识别靶细胞上的MHC I类和肽复合物，并参与疟疾的保护或病理学，我们检查了成红细胞是否被CD 8 + T细胞靶向。用OVA肽脉冲的纯化的未寄生的成红细胞被OVA特异性CD 8 + T细胞识别。重要的是，从GFP-OVA感染的小鼠而不是GFP感染的小鼠分离的寄生的成红细胞活化了OT-I CD 8 + T细胞，表明CD 8 + T细胞以抗原特异性方式识别寄生的成红细胞。
Recent studies show that some human malaria parasite species Plasmodium falciparum and P. vivax parasitize erythroblasts; however, the biological and clinical significance of this is unclear. To investigate further, we generated a rodent malaria parasite (P. yoelii 17XNL) expressing GFP-ovalbumin (OVA). Its infectivity to erythroblasts was confirmed, and parasitized erythroblasts were capable of initiating malaria infections. Experiments showed that MHC class I molecules were highly expressed on parasitized erythroblasts. As CD8+ T cells recognize MHC class I and peptide complexes on target cells, and are involved in protection or pathology against malaria, we examined whether erythroblasts are targeted by CD8+ T cells. Purified non-parasitized erythroblasts pulsed with OVA peptides were recognized by OVA-specific CD8+ T cells. Crucially, parasitized erythroblasts isolated from GFP-OVA-, but not GFP- infected-mice, activated OT-I CD8+ T cells, indicating that CD8+ T cells recognize parasitized erythroblasts in an antigen-specific manner.
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DOI：
10.1182/asheducation-2009.1.87
发表时间：
2009
期刊：
Hematology. American Society of Hematology. Education Program
影响因子：
--
作者：
Haldar K;Mohandas N
通讯作者：
Mohandas N
影响因子：
2.6
作者：
Chen, CY;Pajak, L;Koury, ST
通讯作者：
Koury, ST
影响因子：
6.4
作者：
Lau, Lei Shong;Ruiz, Daniel Fernandez;Heath, William R.
通讯作者：
Heath, William R.
影响因子：
4.4
作者：
Miyakoda, Mana;Kimura, Daisuke;Yui, Katsuyuki
通讯作者：
Yui, Katsuyuki
影响因子：
20.3
作者：
Chang, KH;Tam, MF;Stevenson, MM
通讯作者：
Stevenson, MM