Permethrin Resistance and Target Site Insensitivity in the Mosquito Culex quinquefasciatus in Alabama
Permethrin Resistance and Target Site Insensitivity in the Mosquito Culex quinquefasciatus in Alabama
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DOI：
10.1603/033.046.0625
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发表时间：
2009-11-01
影响因子：
2.1
通讯作者：
Zhang, Lee
中科院分区：
文献类型：
--
作者：
Liu, Nannan;Xu, Qiang;Zhang, Lee
杀虫剂是病媒控制工作中最重要的组成部分，拟除虫菊酯，如氯菊酯，在全世界广泛用于室内控制蚊子。然而，对拟除虫菊酯的抗药性的广泛发展正在成为一个主要问题。目前的研究报告了一项广泛的调查，氯菊酯抗性在亚拉巴马的特点的重要性，拟除虫菊酯抗性库蚊的L-到-F kdr突变。从19个县收集了蚊子，其中17个在亚拉巴马，2个来自佛罗里达和田纳西的邻近县。发现来自所有测试县的致倦库蚊对氯菊酯产生了抗药性。来自亚拉巴马的田间种群中有71%表现出相对较低的抗性水平，抗性比率范围为71至390; 11%具有中/高抗性水平，抗性比率范围为810至830;其余18%具有高抗性水平，抗性比率范围为1，100至1，400。大多数蚊子种群的测试显示出很强的相关性，他们的抗性水平和频率的L-到-F突变的表达，这表明在氯菊酯抗性的发展目标位点不敏感的重要性。然而，耐药水平升高的四个群体在钠通道中没有显示L-至-F突变。虽然它是可能的，其他突变以外的L-到-F是存在于钠通道，导致氯菊酯耐药在这四个领域的人口，耐药机制以外的目标网站不敏感性也必须考虑。
Insecticides are the most important component in the vector-control effort and pyrethroids such its permethrin are widely used for the indoor control of mosquitoes worldwide. However, the widespread development of resistance to pyrethroids is becoming a major problem. The current study reports an extensive survey of permethrin resistance in Alabama designed to characterize the importance of the L-to-F kdr mutation in pyrethroid resistant Culex mosquitoes. Mosquitoes were collected from 19 counties, 17 in Alabama and two from neighboring counties in Florida and Tennessee. Cidex quinquefasciatus Say from all the counties tested were found to have developed resistance to permethrin. Seventy one percent of the field population from Alabama exhibited relatively low levels of resistance, with resistance ratios ranging from 71 to 390; 11% had medium/high levels of resistance, with resistance ratios ranging from 810 to 830; and the remaining 18% had high levels of resistance, with resistance ratios ranging from 1,100 to 1,400. Most of the mosquito populations tested showed a strong correlation between their level of resistance and the frequency of L-to-F mutation expression, suggesting the importance of target site insensitivity in the development of permethrin resistance. However, four populations with elevated levels of resistance showed no L-to-F mutation in their sodium channels. Although it is possible that other mutations other than L-to-F are present in the sodium channel, resulting in permethrin resistance in these four field populations, resistance mechanisms other than target site insensitivity also must be considered.