SBD.4 stimulates regenerative processes in vitro, and wound healing in genetically diabetic mice and in human skin/severe-combined immunodeficiency mouse chimera.
SBD.4 stimulates regenerative processes in vitro, and wound healing in genetically diabetic mice and in human skin/severe-combined immunodeficiency mouse chimera.
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SBD.4 可刺激遗传性糖尿病小鼠和人类皮肤/严重联合免疫缺陷小鼠嵌合体的体外再生过程和伤口愈合。
DOI：
10.1111/j.1743-6109.2006.00166.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Bojanowski,Krzysztof
中科院分区：
文献类型：
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作者：
Zhao,Hui;Mortezaei,Reza;Wang,Yanrong;Sheng,Xinsheng;Aria,Fariba;Bojanowski,Krzysztof
为了寻找新的血管刺激剂，我们对有益于血液循环的药用植物进行了高通量筛选。从阳性结果中选择当归作为研究对象。纯化至低分子量部分并通过高效液相色谱-质谱法表征，该材料命名为SBD. 4A，在糖尿病小鼠伤口愈合模型和人/严重联合免疫缺陷小鼠嵌合体伤口愈合模型中显示出特别强的伤口愈合活性。在这两种模型中，SBD. 4A与美国食品和药物管理局批准的伤口愈合药物becaplermin相比，这表明这种植物产品可能是难以愈合伤口的有价值的治疗方法。SBD. 4A的进一步高效液相色谱分级分离产生亲水性级分，其强烈刺激内皮细胞增殖、三维内皮细胞网络形成以及人真皮成纤维细胞增殖和I型胶原分泌。由于血管生成和成纤维细胞增殖对伤口愈合至关重要，我们提出这种液相色谱-质谱法定义的亲水性部分至少部分负责SBD. 4A的伤口愈合活性。
In search of novel angiostimulators, we performed a high‐throughput screening of medicinal plants beneficial for blood circulation. From the panel of positive hits,Angelica sinensiswas selected for further investigation. Purified down to a low‐molecular‐weight fraction and characterized by high‐performance liquid chromatography–mass spectrometry, the material, named SBD.4A, revealed a particularly strong wound healing activity in the diabetic mouse wound‐healing model, and in the human/severe combined immunodeficiency mouse chimera wound‐healing model. In both models, SBD.4A compared favorably with the Food and Drug Administration‐approved wound‐healing drug becaplermin, suggesting that this botanical product could be a valuable treatment for difficult‐to‐heal wounds. Further high‐performance liquid chromatography fractionation of SBD.4A yielded a hydrophilic fraction, which strongly stimulated endothelial cell proliferation, tridimensional endothelial cell network formation, as well as the proliferation of human dermal fibroblasts and type I collagen secretion. Because angiogenesis and fibroblast proliferation are essential for wound healing, we propose that this liquid chromatography–mass spectrometry‐defined hydrophilic fraction is at least partially responsible for the wound‐healing activity of SBD.4A.