Evaluation of Urea Cycle Activity by Metabolic Flux Analysis Using Mass Spectrometry
Evaluation of Urea Cycle Activity by Metabolic Flux Analysis Using Mass Spectrometry
复制标题
使用质谱法通过代谢通量分析评估尿素循环活性
DOI：
10.1007/978-1-0716-2557-6_9
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Taniguchi Hideki
中科院分区：
文献类型：
--
作者：
Ueno Yasuharu;Maeda Takuji;Okamoto Satoshi;Taniguchi Hideki
肝细胞通过执行各种代谢功能，在维持生物体内的稳态方面发挥着重要作用。尿素循环是肝细胞代谢途径之一，是将有毒氨转化为无毒尿素的重要代谢途径。使用质谱仪对单个代谢物水平进行定量评估可用于评估肝细胞中尿素循环的代谢状态。此外，使用稳定同位素和质谱仪的代谢通量分析是用于测量代谢状态的新技术。它能够对目标代谢途径的激活状态进行特异性、客观性和定量测量，而不管外部干扰因素。本节描述了通过质谱法进行尿素循环的代谢通量分析的技术背景和方法。
Hepatocytes play an important role in maintaining homeostasis in living organisms by carrying out various metabolic functions. The urea cycle, one of the metabolic pathways taking place in hepatocytes, is an important metabolic pathway that converts toxic ammonia to nontoxic urea. Performing quantitative assessments of individual metabolite levels using a mass spectrometer is useful for assessing the metabolic state of the urea cycle in hepatocytes. In addition, metabolic flux analysis using stable isotopes and a mass spectrometer is a new technique for measuring the metabolic state. It enables conducting specific, objective, and quantitative measurement of the activated state of the target metabolic pathway regardless of external disturbing factors. This section describes the technical background and methodology of performing metabolic flux analysis of the urea cycle by mass spectrometry.