Food additive-induced oxidative stress in rat male reproductive organs and hippocampus
Food additive-induced oxidative stress in rat male reproductive organs and hippocampus
复制标题
食品添加剂诱导大鼠雄性生殖器官和海马氧化应激
DOI：
10.1016/j.abb.2021.108810
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发表时间：
2021
影响因子：
3.9
通讯作者：
Ichikawa Hiroshi
中科院分区：
文献类型：
--
作者：
Minamiyama Yukiko;Takemura Shigekazu;Ichikawa Hiroshi
按照目前的定义，暴露组代表了个体一生中对所有潜在的外部遗传影响及其对健康的影响的暴露程度。尽管有意添加的化学品（例如食品添加剂）和食品接触材料（例如包装、杀虫剂）已在一定程度上进行了安全评估，但它们对健康和生殖的影响程度尚未得到报道。本研究的目的是确定食品添加剂对雄性大鼠大脑和精子/睾丸的体外和体内影响，特别是通过氧化应激。我们的体外研究结果表明，使用常见食品添加剂甜菊糖（常见甜味剂甜菊糖的主要成分）以及防腐剂二苯基和邻苯基苯酚（OPP）会诱导精子产生活性氧（ROS），并导致精子功能障碍。添加抗氧化剂 α-生育酚可抑制这些影响。此外，OPP治疗（未观察到不良反应的1/10,000）在体内两周后诱导精子中ROS的产生以及附睾和海马中的脂质过氧化。此外，在睾丸精母细胞和大脑齿状回颗粒细胞层中检测到 4-羟基壬烯醛阳性细胞，表明 ROS 产生的蛋白质修饰。 α-生育酚治疗显着改善氧化应激。我们的研究表明，某些食品添加剂可能会影响精子功能并诱发睾丸和大脑氧化应激，导致不孕和短期记忆丧失，而一些抗氧化剂可能会改善这些功能障碍。
As currently defined, the exposome represents the lifetime exposure measure of an individual to all potential external genetic influences and their impact on health. Although intentionally added chemicals (e.g., food additives) and food contact materials (e.g., packaging, pesticides) have been assessed for safety to some degree, the full extent to which they can affect health and reproduction has not been reported. The aim of this study was to determine thein vitroandin vivoeffects of food additives on the male rat brain and sperm/testes, particularly through oxidative stress. Results from ourin vitrostudy demonstrated that the administration of the common food additive, stevioside, a major component of the common sweetener stevia, as well as the preservatives, diphenyl and orthophenyl phenol (OPP), induced reactive oxygen species (ROS) production in sperm, and led to sperm dysfunction. These effects were inhibited by the addition of the antioxidant α-tocopherol. Moreover, OPP treatment (1/10,000 of no observed adverse effect) induced ROS production in sperm and lipid peroxidation in the epididymis and hippocampus after two weeksin vivo. Furthermore, 4-hydroxynonenal-positive cells, indicating ROS-generated protein modifications, were detected in spermatocytes in the testes and granular cell layer of the dentate gyrus in the brain. Treatment with α-tocopherol significantly improved oxidative stress. Our study suggests that certain food additives may affect sperm function and induce oxidative stress in the testes and brain, resulting in infertility and short-term memory loss, and some antioxidants may improve these dysfunctions.