New insights into the effect of NdhO levels on cyanobacterial cell death triggered by high temperature
New insights into the effect of NdhO levels on cyanobacterial cell death triggered by high temperature
复制标题
关于 NdhO 水平对高温引发的蓝藻细胞死亡影响的新见解
DOI：
10.1071/fp21097
复制
发表时间：
2021
影响因子：
3
通讯作者：
Ma Weimin
中科院分区：
文献类型：
--
作者：
Zhao Jiaohong;Jiang Yuanyuan;Tian Yuhao;Mao Jun;Wei Lanzhen;Ma Weimin
NdhO是一种光合作用的特异性调节亚基，靠近蓝藻NDH-1的铁氧还蛋白结合位点，其水平与NDH-1介导的PSI周围的循环电子传递速率（NDH-CET）呈负相关。然而，NdhO水平对高温引发的蓝藻细胞死亡的影响仍然难以捉摸。在这里，我们的结果揭示了当将在30°C下生长1天的蓝藻细胞转移到45°C下2天时，NdhO水平对细胞死亡和活性氧（ROS）积累的协同效应。这种协同效应与高温下NDH-CET和CO2同化活动密切相关。总之，我们认为NdhO水平对高温引发的蓝藻细胞漂白和细胞死亡的影响是影响NDH-CET产生ROS的结果，NDH-CET被认为对平衡ATP/NADPH比率和改善Calvin-Benson循环至关重要。
NdhO, a regulatory oxygenic photosynthesis-specific subunit, is close to the ferredoxin-binding site of cyanobacterial NDH-1, and its levels are negatively associated with the rates of cyclic electron transfer around PSI mediated by NDH-1 (NDH-CET). However, the effect of NdhO levels on cyanobacterial cell death triggered by high temperature remains elusive. Here, our results uncovered a synergistic effect of NdhO levels on the cell death and reactive oxygen species (ROS) accumulation when cyanobacterial cells grown at 30°C for 1 day were transferred to 45°C for 2 days. Such synergistic effect was found to be closely associated with the activities of NDH-CET and CO2 assimilation during high temperature. Collectively, we propose that the effect of NdhO levels on the cyanobacterial cell bleaching and cell death triggered by high temperature is a result of influencing production of ROS by NDH-CET, which is considered to be vital to balance the ATP/NADPH ratio and improve the Calvin-Benson cycle.