Stylet penetration of Cacopsylla pyri; an electrical penetration graph (EPG) study
Stylet penetration of Cacopsylla pyri; an electrical penetration graph (EPG) study
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DOI：
10.1016/j.jinsphys.2011.07.008
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发表时间：
2011-10-01
影响因子：
2.2
通讯作者：
Tjallingii, W. Freddy
中科院分区：
文献类型：
--
作者：
Civolani, Stefano;Leis, Marilena;Tjallingii, W. Freddy
梨木虱Cacopsyllapyri L（半翅目木虱科）侵入植物的详细信息是必要的，以研究植原体传播的“梨植原体”负责梨衰退病（PD），跟踪和评价新的梨树选择先进的育种计划的抗性性状。电穿透图技术或（全）EPG可能有助于这方面的知识。C. Pyri EPG波形的特征在于振幅，频率，电压电平和电起源的基础上。此外，探针轨道和推定的位置探针尖端在植物组织中的组织学相关的EPG波形的光和透射电子显微镜观察后花柱切除术。在同一组织中，PA、PB、PC 1和PC 2均出现在叶肉中，PE 1和PE 2均出现在韧皮部中。波形PE 1总是在瞬态波形PD之前，如先前在其他木虱中所述。有趣的是，在植物渗透过程中没有观察到短暂的细胞内穿刺（电位下降波形），与蚜虫和其他Sternorrhyncha中通常记录的相反。(C)2011爱思唯尔有限公司保留所有权利。
Detailed information on plant penetration activities by pear psylla Cacopsylla pyri L (Hemiptera Psyllidae) is essential to study phytoplasma transmission of "Candidatus Phytoplasma pyri" responsible of pear decline disease (PD) and to trace and evaluate resistant traits in new pear tree selections for advanced breeding programs. The electrical penetration graph technique or (full) EPG may relevantly contribute to this knowledge. C. pyri EPG waveforms were characterized on basis of amplitude, frequency, voltage level, and electrical origin. Additionally, stylet tracks and the putative location of stylet tips in the plant tissue were histologically related to EPG waveforms by light and transmission electron microscopy observations after stylectomy. More than one waveform occurred in the same tissue: PA, PB, PC1 and PC2 were all detected in the mesophyll, and PE1 and PE2 were both recorded in the phloem. Waveform PE1 was always preceded by transient waveform PD, as previously described in other psyllids. Interestingly, no brief intracellular punctures (potential drop waveforms) were observed during plant penetration, opposite of what is usually recorded in aphids and other Sternorrhyncha. (C) 2011 Elsevier Ltd. All rights reserved.