Separate urinary bladder and external urethral sphincter neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses
Separate urinary bladder and external urethral sphincter neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses
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DOI：
10.1016/s0006-8993(01)02349-6
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发表时间：
2001-06-08
期刊：
影响因子：
2.9
通讯作者：
Vera, PL
中科院分区：
文献类型：
--
作者：
Nadelhaft, I;Vera, PL
这项工作检查的分布，在中枢神经系统中，病毒标记的神经元从大鼠膀胱和尿道外括约肌同时在同一组织切片。将两种化学上不同的伪狂犬病病毒株注射到雄性Sprague-Dawley大鼠（类似于280 g）中。一种病毒被注入膀胱，另一种注入尿道外括约肌。在2、2.5和3天的孵育间隔后，对脊髓和脑的切片进行免疫化学处理，以检测分别被每种病毒标记或被两种病毒标记的细胞。这些实验的主要结果是，每种病毒株标记一个单独的群体的神经元，一些神经元被两种病毒株标记，在腰骶髓，感染后3天，神经元标记的病毒从尿道外括约肌被发现在Onuf的核，背侧灰质连合，和浅背角。在L 6-S1和L1-L2脊髓节段的背侧灰质连合、中间外侧区和浅背角内发现了来自膀胱的病毒标记的神经元。双标中间神经元主要分布在背侧灰质连合，中间外侧区和背角浅层也有分布。在延髓，尿道外括约肌神经元和膀胱神经元以及双标神经元分布于网状区和中缝。更喙，膀胱神经元位于脑桥排尿中心和外尿道括约肌神经元被发现在蓝斑和蓝斑下。在脑桥排尿中枢和蓝斑或蓝斑下核内发现极少量的双标神经元。
This work examines the distribution, in the central nervous system, of virus-labeled neurons from the rat urinary bladder and the external urethral sphincter simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were injected into male Sprague-Dawley rats (similar to 280 g). One virus was injected into the bladder and the other into the external urethral sphincter. After incubation intervals of 2, 2.5 and 3 days, sections from the spinal cord and brain were treated immunohistochemically to detect cells which were labeled separately by each virus or were labeled by both viruses. The major result of these experiments is that each strain of virus labeled a separate population of neurons and that some neurons were labeled by both strains, in the lumbosacral cord, 3 days post-infection, neurons labeled by virus from the external urethral sphincter were found in Onuf's nucleus, the dorsal gray commissure, and the superficial dorsal horn. Neurons labeled by virus from the urinary bladder were found in the L6-S1 and L1-L2 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Double-labeled interneurons were mainly located in the dorsal gray commissure although some were also found in the intermediolateral area and the superficial dorsal horn. In the medulla, external urethral sphincter neurons and bladder neurons and double-labeled neurons were found in the reticular region and the raphe. More rostrally, bladder neurons were located in the pontine micturition center and external urethral sphincter neurons were found in the locus coeruleus and subcoeruleus. A very small number of double-labeled neurons were found in the pontine micturition center and the locus coeruleus or subcoeruleus.