High-sensitivity mass spectrometry for imaging subunit interactions: hydrogen/deuterium exchange
High-sensitivity mass spectrometry for imaging subunit interactions: hydrogen/deuterium exchange
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DOI：
10.1016/j.sbi.2004.03.006
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发表时间：
2004-04-01
影响因子：
6.8
通讯作者：
Prevelige, PE
中科院分区：
文献类型：
--
作者：
Lanman, J;Prevelige, PE
近年来，质谱技术的进步为细胞内蛋白质表达提供了前所未有的知识。很明显，许多蛋白质的功能都是大分子复合物。结构基因组学计划正在以越来越快的速度确定这些蛋白质的折叠，电子显微镜断层扫描可能提供了一种确定这些复合物在细胞内位置的方法。要全面了解这些蛋白质的分子机制，需要有关复合物内相互作用和动力学的详细信息。质谱的最新进展，现在有可能使用氢/氘交换来检测亚基间的接口和超分子复合物内的动力学。
In recent years, advances in mass spectrometry have provided unprecedented knowledge of protein expression within cells. It has become apparent that many proteins function as macromolecular complexes. Structural genomics programs are determining the fold of these proteins at an increasing rate and electron microscopic tomography potentially provides a means to determine the location of these complexes within the cell. A complete understanding of the molecular mechanism of these proteins requires detailed information on the interactions and dynamics within the complex. Recent advances in mass spectrometry now make it possible to use hydrogen/deuterium exchange to detect intersubunit interfaces and dynamics within supramolecular complexes.