Cellular construction of a circadian clock: Period determination in the suprachiasmatic nuclei
Cellular construction of a circadian clock: Period determination in the suprachiasmatic nuclei
复制标题
DOI：
10.1016/s0092-8674(00)80473-0
复制
发表时间：
1997-12-12
期刊：
影响因子：
64.5
通讯作者：
Reppert, SM
中科院分区：
文献类型：
--
作者：
Liu, C;Weaver, DR;Reppert, SM
视交叉上核中的昼夜节律钟由多个单细胞昼夜节律振荡器（钟细胞）组成。我们现在检验这样的假设：行为的昼夜节律周期是由时钟细胞与不同昼夜节律周期耦合产生的平均周期决定的。在这些研究中，我们在固定的多电极板上监测单个视交叉上核神经元的放电率节律，并利用杂合和纯合tau突变仓鼠表达的改变的昼夜节律。结果表明，在整个动物的昼夜节律周期是由平均广泛分散的单个时钟细胞的周期。数据还表明，tau突变以细胞自主的方式影响昼夜节律功能。
The circadian clock in the suprachiasmatic nuclei is composed of multiple, single-cell circadian oscillators (clock cells). We now test the hypothesis that the circadian period in behavior is determined by the mean period that arises from the coupling of clock cells with diverse circadian periods. For these studies, we monitored firing rate rhythms of individual suprachiasmatic nuclei neurons on fixed multielectrode plates and exploited the altered circadian periods expressed by heterozygous and homozygous tau mutant hamsters. The results show that circadian period in the whole animal is determined by averaging widely dispersed periods of individual clock cells. The data also demonstrate that the tau mutation affects circadian function in a cell-autonomous manner.