A COMPARISON OF BATHOPHENANTHROLINEDISULFONIC ACID AND FERROZINE AS CHELATORS OF IRON(II) IN REDUCTION REACTIONS
A COMPARISON OF BATHOPHENANTHROLINEDISULFONIC ACID AND FERROZINE AS CHELATORS OF IRON(II) IN REDUCTION REACTIONS
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DOI：
10.1006/abio.1993.1246
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发表时间：
1993-05-15
影响因子：
2.9
通讯作者：
HIND, JS
中科院分区：
文献类型：
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作者：
COWART, RE;SINGLETON, FL;HIND, JS
红菲咯啉二磺酸（4,7-二苯基-1,10-菲咯啉二磺酸 [BPS]）和 3-(2-吡啶基)-5,6-双(4-苯基磺酸)-1,2,4-三嗪（铁嗪）是亚铁螯合剂，通常用于测定各种样品中的铁 (II) 浓度以及识别或测量生物系统中的铁还原。在本研究中，确定了铁嗪和 BPS 螯合由选定还原剂从 FeS3+-配体还原的铁 (II) 的功效。我们的结果表明 (i) BPS 和铁嗪作为铁还原活性的动力学指标并不等同； (ii) 二硫苏糖醇还原 Fe3+-配体的表观初始速率，如铁 (II) 与 BPS 或铁嗪形成复合物所示，相差 50 倍； (iii) BPS 和铁嗪均发生一些 Fe3+-配体的非特异性还原。在相同条件下，Fe2+-铁嗪的形成速率通常慢于Fe2+-BPS的形成速率。这些数据表明，在使用 BPS 或铁嗪监测铁还原动力学的任何实验设计中都应仔细考虑。
Bathophenanthrolinedisulfonic acid (4,7-diphenyl-1,10-phenanthrolinedisulfonic acid [BPS]) and 3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine (ferrozine), chelators of ferrous iron, are often used to determine iron(II) concentrations in various samples and for identifying or measuring iron reduction in biological systems. In this study, the efficacy of ferrozine and BPS to chelate iron(II) reduced from FeS3+-ligands by selected reducing agents was determined. Our results indicate that (i) BPS and ferrozine are not equivalent as kinetic indicators of iron reducing activity; (ii) apparent initial rates of reduction of Fe3+-ligands by dithiothreitol, as indicated by formation of complexes of iron(II) with either BPS or ferrozine, differed by a factor of 50; and (iii) nonspecific reduction of some Fe3+-ligands by both BPS and ferrozine occurred. Under identical conditions, rates of formation of Fe2+-ferrozine generally were slower than rates of formation of Fe2+-BPS. These data suggest careful consideration should be given in the design of any experiments where kinetics of iron reduction are monitored with BPS or ferrozine.