The miR172 target TOE3 represses AGAMOUS expression during Arabidopsis floral patterning
The miR172 target TOE3 represses AGAMOUS expression during Arabidopsis floral patterning
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DOI：
10.1016/j.plantsci.2013.10.010
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发表时间：
2014-02-01
期刊：
影响因子：
5.2
通讯作者：
Park, Chung-Mo
中科院分区：
文献类型：
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作者：
Jung, Jae-Hoon;Lee, Sangmin;Park, Chung-Mo
microRNA 172（miR 172）通过抑制编码APETALA 2（AP 2）和AP 2样转录因子的靶标来调节拟南芥中的相变和花图案形成。miR 172介导的AP 2基因抑制限制AGAMOUS（AG）表达。此外，大多数miR 172靶标，包括AP 2，冗余地充当成花阻遏物，并且靶基因的过表达导致延迟开花。然而，miR 172靶点而不是AP 2如何调节这两个发育过程仍不清楚。在这里，我们证明了miR 172介导的抑制目标EAT 3（TOE 3）基因是至关重要的花模式在拟南芥。过表达miR 172抗性TOE 3基因（rTOE 3-ox）的转基因植物表现出具有大量雄蕊和心皮状器官的不定花，这与先前在过表达miR 172抗性AP 2基因的转基因植物中的观察结果一致。TOE 3与AG基因的第二个内含子结合。因此，AG表达在rTOE 3-ox植物中显著降低。TOE 3还与细胞核中的AP 2相互作用。鉴于AP 2在花图案形成中的主要作用，miR 172可能至少部分地通过AP 2调节花图案形成中的TOE 3。此外，miR 156靶向的SQUAMOSA启动子结合蛋白样3直接激活TOE 3表达，揭示了miR 156和miR 172在花图案形成中的新型信号相互作用。(C)2013爱思唯尔爱尔兰有限公司版权所有。
microRNA172 (miR172) regulates phase transition and floral patterning in Arabidopsis by repressing targets that encode the APETALA2 (AP2) and AP2-like transcription factors. The miR172-mediated repression of the AP2 gene restricts AGAMOUS (AG) expression. In addition, most miR172 targets, including AP2, redundantly act as floral repressors, and the overexpression of the target genes causes delayed flowering. However, how miR172 targets other than AP2 regulate both of the developmental processes remains unclear. Here, we demonstrate that miR172-mediated repression of the TARGET OF EAT 3 (TOE3) gene is critical for floral patterning in Arabidopsis. Transgenic plants that overexpress a miR172-resistant TOE3 gene (rTOE3-ox) exhibit indeterminate flowers with numerous stamens and carpelloid organs, which is consistent with previous observations in transgenic plants that overexpress a miR172-resistant AP2 gene. TOE3 binds to the second intron of the AG gene. Accordingly, AG expression is significantly reduced in rTOE3-ox plants. TOE3 also interacts with AP2 in the nucleus. Given the major role of AP2 in floral patterning, miR172 likely regulates TOE3 in floral patterning, at least in part via AP2. In addition, a miR156 target SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 directly activates TOE3 expression, revealing a novel signaling interaction between miR156 and miR172 in floral patterning. (C) 2013 Elsevier Ireland Ltd. All rights reserved.