Rice BIG gene is required for seedling viability
Rice BIG gene is required for seedling viability
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水稻BIG基因是幼苗活力所必需的
DOI：
10.1016/j.jplph.2018.11.006
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Liang, Yun-Kuan
中科院分区：
文献类型：
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作者：
Cheng, Rui;Gong, Luping;Liang, Yun-Kuan
拟南芥BIG(AtBIG)基因编码生长素运输所需的大量蛋白质，AtBIG功能的丧失不仅深刻改变植物的结构，还改变植物对环境刺激的适应性。在水稻基因组中存在AtBIG的假定同源基因，但尚未将其功能归因于它。在本研究中，我们重点研究了OsBIG的基因结构和功能。序列和系统发育分析表明，OsBIG的同源物在多个区域具有高度的跨物种氨基酸保守性。通过CRISPR/Cas9系统介导的基因组编辑干扰OsBIG表达的转基因水稻植株用于表型分析。OsBig/-植株表现出较高的细胞死亡水平，电解质渗漏和膜脂过氧化作用增强，叶绿素含量降低，这可能是导致幼苗死亡的原因。此外，RNA-seq分析表明，OsBIG缺乏显著干扰了一些代谢和激素途径，包括核糖体、DNA复制、光合作用和叶绿素代谢。综上所述，OsBIG基因对水稻的正常生长发育是不可或缺的。
Arabidopsis BIG (AtBIG) gene encodes an enormous protein that is required for auxin transport Loss of AtBIG function not only profoundly changes plant architecture but also alters plant adaptability to environmental stimuli. A putative homolog of AtBIG exists in the rice genome, but no function has been ascribed to it. In this study, we focus on the characterization of the gene structure and function of OsBIG. Sequence and phylogenetic analysis shows that the homologs of OsBIG have high amino acid conservation in several domains across species. Transgenic rice plants in which the expression of OsBIG was disrupted through the CRISPR/Cas9 system-mediated genome editing were used for phenotypic analysis. The Osbig/- plants show high levels of cell death, enhanced electrolyte leakage and membrane lipid peroxidation, and reduced chlorophyll content, which likely accounted for the seedling lethality. Moreover, gene expression between Osbig/- and wild-type plants analyzed by RNA-seq indicates that a number of metabolic and hormonal pathways including ribosome, DNA replication, photosynthesis, and chlorophyll metabolism were significantly perturbed by OsBIG deficiency. In summary, OsBIG gene is integral to the normal growth and development in rice.