OLIGONUCLEOTIDE-POLYAMIDE HYBRID MOLECULES CONTAINING MULTIPLE PYRENE RESIDUES EXHIBIT SIGNIFICANT EXCIMER FLUORESCENCE
OLIGONUCLEOTIDE-POLYAMIDE HYBRID MOLECULES CONTAINING MULTIPLE PYRENE RESIDUES EXHIBIT SIGNIFICANT EXCIMER FLUORESCENCE
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DOI：
10.1021/ja00154a015
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发表时间：
1995-12-13
影响因子：
15
通讯作者：
HARALAMBIDIS, J
中科院分区：
文献类型：
--
作者：
TONG, G;LAWLOR, JM;HARALAMBIDIS, J
制备了聚酰胺部分含有多个芘残基的寡核苷酸-聚酰胺杂化分子。这些分子的设计目的是促进芘准分子的形成，避免标记插入DNA双链。观察到了显著的准分子荧光，并且这被证明是随着存在的芘残基的数量而增加的。因此，有可能通过加入额外的标记残基来进一步增加荧光强度。激基缔合物的荧光强度对双链形成也很敏感。荧光测量和熔融温度研究没有证据表明芘残留物插入到双链DNA中。这些杂化分子的杂交性质与未修饰的寡核苷酸相似。因此，可能的情况是，丙二烯基化的寡核苷酸-聚酰胺杂交物可能用作寡核苷酸探针。
Oligonucleotide-polyamide hybrid molecules bearing multiple pyrene residues in the polyamide moiety were prepared. These molecules were designed to promote pyrene excimer formation and avoid intercalation of the label into the DNA duplex. Significant excimer fluorescence was observed, and this was shown to increase with the number of pyrene residues present. Hence, there is potential for further increasing of the fluorescence intensity by incorporation of additional label residues. Excimer fluorescence intensity was also shown to be sensitive to duplex formation. Fluorescence measurements and melting temperature studies gave no evidence of intercalation of the pyrene residues into duplex DNA. The hybridization properties of these hybrid molecules are similar to those of unmodified oligonucleotides. It seems, then, likely that pyrenylated oligonucleotide-polyamide hybrids may be useful as oligonucleotide probes.