Localization of cystic fibrosis transmembrane conductance regulator signaling complexes in human salivary gland striated duct cells.
Localization of cystic fibrosis transmembrane conductance regulator signaling complexes in human salivary gland striated duct cells.
复制标题
囊性纤维化跨膜电导调节信号复合物在人唾液腺横纹管细胞中的定位。
DOI：
10.1111/eos.12184
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发表时间：
2015
影响因子：
1.9
通讯作者：
Hand,ArthurR
中科院分区：
文献类型：
--
作者：
Zinn,VinaZ;Khatri,Aditi;Mednieks,MaijaI;Hand,ArthurR
The cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic AMP‐dependent protein kinase (PKA)‐regulated Cl−channel, crucial for epithelial cell regulation of salt and water transport. Previous studies showed that ezrin, an actin binding and A‐kinase anchoring protein (AKAP), facilitates association of PKA with CFTR. We used immunohistochemistry and immunogold transmission electron microscopy to localize CFTR, ezrin, and PKA type II regulatory (RII) and catalytic (C) subunits in striated duct cells of human parotid and submandibular glands. Immunohistochemistry localized the four proteins mainly to the apical membrane and the apical cytoplasm of striated duct cells. In acinar cells, ezrin localized to the luminal membrane, and PKA RII subunits were present in secretory granules, as previously described. Immunogold labeling showed that CFTR and PKA RII and C subunits were localized to the luminal membrane and associated with apical granules and vesicles of striated duct cells. Ezrin was present along the luminal membrane, on microvilli and along the junctional complexes between cells. Double labeling showed specific protein associations with apical granules and vesicles and along the luminal membrane. Ezrin, CFTR, and PKA RII and C subunits are co‐localized in striated duct cells, suggesting the presence of signaling complexes that serve to regulate CFTR activity.
