Molecular phylogenetics of subtribe Aeridinae (Orchidaceae):: insights from plastid matK and nuclear ribosomal ITS sequences
Molecular phylogenetics of subtribe Aeridinae (Orchidaceae):: insights from plastid matK and nuclear ribosomal ITS sequences
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DOI：
10.1007/s10265-005-0217-3
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发表时间：
2005-08-01
影响因子：
2.8
通讯作者：
Ito, M
中科院分区：
文献类型：
--
作者：
Hidayat, T;Yukawa, T;Ito, M
我们进行了系统发育分析，使用两个DNA序列数据集来自matK，成熟酶编码基因位于一个内含子的质体基因trnK，和内部转录间隔区的18 S-26 S核核糖体DNA，以研究在亚族Aeridinae（兰科）的关系。具体而言，我们调查了（1）亚族中属间的系统发育关系，（2）以前的亚族分类与分子数据推断的系统发育关系之间的一致性，以及（3）亚族分类学上重要特征的进化趋势，如花粉块，距唇和柱足。共调查到气虫亚科62属75种。分析结果表明：（1）该亚族的单系性得到初步支持，其中可识别出14个反映系统发育关系的亚支;（2）该亚族的分类结果与以往的分类结果不一致;（3）先前强调的特征如花粉块数目和孔、距长和柱足等的重复进化得到证实。结果表明，这些特征的相似性是造成这些不一致的主要原因。在属的水平上，蝴蝶兰属、隔唇兰属和肉唇兰属是非单系的。
We conducted phylogenetic analyses using two DNA sequence data sets derived from matK, the maturase-coding gene located in an intron of the plastid gene trnK, and the internal transcribed spacer region of 18S-26S nuclear ribosomal DNA to examine relationships in subtribe Aeridinae (Orchidaceae). Specifically, we investigated (1) phylogenetic relationships among genera in the subtribe, (2) the congruence between previous classifications of the subtribe and the phylogenetic relationships inferred from the molecular data, and (3) evolutionary trends of taxonomically important characters of the subtribe, such as pollinia, a spurred lip, and a column foot. In all, 75 species representing 62 genera in subtribe Aeridinae were examined. Our analyses provided the following insights: (1) monophyly of subtribe Aeridinae was tentatively supported in which 14 subclades reflecting phylogenetic relationships can be recognized, (2) results are inconsistent with previous classifications of the subtribe, and (3) repeated evolution of previously emphasized characters such as pollinia number and apertures, length of spur, and column foot was confirmed. It was found that the inconsistencies are mainly caused by homoplasy of these characters. At the genus level, Phalaenopsis, Cleisostoma, and Sarcochilus are shown to be non-monophyletic.