Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold
Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold
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DOI：
10.1021/ja049956q
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发表时间：
2004-04-21
影响因子：
15
通讯作者：
Chilkoti, A
中科院分区：
文献类型：
--
作者：
Hyun, J;Kim, J;Chilkoti, A
本文描述了一种简单的策略，利用直接构图在呈现末端寡核苷酸的自组装单分子层上的核酸内切酶(DNase I)，通过酶浸笔纳米光刻技术在纳米尺度上对表面进行生化操作。由于酶与固定的寡核苷酸底物的亲和力，物理吸附的DNase I纳米分子在表面进行了纳米尺度的酶学研究，创建了具有图案酶保真度的寡核苷酸图案。
This paper describes a simple strategy to biochemically manipulate a surface at the nanoscale by enzyme dip-pen nanolithography using an endonuclease (DNase I) that is directly patterned on a self-assembled monolayer presenting a terminal oligonucleotide. Physisorbed nanopatterns of DNase I carried out nanoscale enzymology at the surface creating oligonucleotide patterns with the fidelity of the patterned enzyme because of the affinity of the enzyme for the immobilized, oligonucleotide substrate.