TAXONOMIC STUDY OF METHANOL-ASSIMILATING YEASTS

TAXONOMIC STUDY OF METHANOL-ASSIMILATING YEASTS
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甲醇同化酵母的分类学研究

DOI:
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发表时间:
1980
期刊:
影响因子:
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通讯作者:
K. Komagata
K. Komagata
中科院分区:
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文献类型:
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作者:
Jae;K. Komagata

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对31株甲醇同化酵母菌进行了初步鉴定,其中纤维毕赤酵母3株,博伊丁假丝酵母20株,卡氏木质假丝酵母1株,嗜succiphila假丝酵母6株,毛球拟酵母1株。Kloeckera sp.No.2201和Candida methanolica,以及Candida koshuensis重新鉴定为C. Boidinii的生物学和化学分类学特征。产甲醇球拟酵母与林德纳毕赤酵母具有相似的特征,两者的化学分类学特征一致。这些酵母的DNA碱基组成(GC含量的DNA)分布在29.2%至50.2%的广泛范围内。泛醌系统,细胞壁甘露聚糖的质子磁共振光谱,和甲醇氧化酶的电泳模式为每个物种的特征。这些甲醇同化酵母可分为四类:1)C. boidinii类群; 2)甲醇同化汉逊酵母及其近缘酵母; 3)纤维双孢酵母类群; 4)荚膜汉逊酵母类群。
Thirty-one freshly isolated strains of methanol-assimilating yeasts were identified as Pichia cellobiosa (3 strains), Candida boidinii (20 strains), Candida cariosilignicola (1 strain), Candida succiphila (6 strains), and Torulopsis molischiana (1 strain). Kloeckera sp. No. 2201 and Candida methanolica, which were reported as methanol-assimilating yeasts, and Candida koshuensis were reidentified as C. boidinii on the basis of biological and chemotaxonomic characteristics. Torulopsis methanolovescens showed characteristics similar to those of Pichia lindnerii, and the chemotaxonomic characteristics of the two were in good agreement. The DNA base composition (GC-content of DNA) of these yeasts was distributed in a broad range from 29.2% to 50.2%. The ubiquinone system, proton magnetic resonance spectra of cell-wall mannans, and electrophoretic patterns of methanol oxidase were characteristic for each species. These methanol-assimilating yeasts were divided into four groups: 1) the C. boidinii group, 2) methanol-assimilating Hansenula and its closely related yeasts, 3) the P. cellobiosa group, and 4) the Hansenula capsulata group.