Vitellogenin family gene expression does not increase Drosophila lifespan or fecundity.
Vitellogenin family gene expression does not increase Drosophila lifespan or fecundity.
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DOI：
10.12688/f1000research.3975.1
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hughes KA
中科院分区：
文献类型：
--
作者：
Ren Y;Hughes KA
比较生物学中最引人注目的模式之一是在物种之间的比较中观察到的寿命和繁殖力之间的负相关性。这种模式与生物体需要在生长、发育、繁殖和体细胞维持的竞争需求之间分配固定能量预算的想法一致。然而，在许多社会性昆虫中观察到了这种模式的例外，包括蚂蚁、蜜蜂和白蚁。在蜜蜂（Apis mellifera）中，卵黄蛋白原（Vg），一种卵黄蛋白前体，被认为与蜂王的长寿和高繁殖力有关。为了确定Vg样蛋白是否可以调节昆虫的寿命，我们使用RU 486诱导的GeneSwitch系统研究了Apis Vg和果蝇CG 31150（最近被鉴定为cv-d的Vg样基因）的表达对果蝇寿命和繁殖力的影响。对于所有的基因型测试，Vg和CG 31150的过表达降低果蝇寿命，并没有影响总的或年龄特异性的繁殖力。我们还检测到GeneSwitch系统本身的明显作用，其中RU 486暴露（或其诱导的GAL 4表达）导致我们的果蝇品系中寿命显著增加和繁殖力降低。这一结果与最近对果蝇衰老研究的荟萃分析中报道的模式一致，其中UAS/GAL 4表达系统的转基因构建体在某些遗传背景下应该没有影响（例如未诱导的GeneSwitch），但显著延长了寿命。我们的研究结果表明，Vg家族基因是不是果蝇生活史性状的主要调节因子，并强调了在衰老研究中使用适当控制的重要性。
One of the most striking patterns in comparative biology is the negative correlation between lifespan and fecundity observed in comparisons among species. This pattern is consistent with the idea that organisms need to allocate a fixed energy budget among competing demands of growth, development, reproduction and somatic maintenance. However, exceptions to this pattern have been observed in many social insects, including ants, bees, and termites. In honey bees ( Apis mellifera), Vitellogenin ( Vg), a yolk protein precursor, has been implicated in mediating the long lifespan and high fecundity of queen bees. To determine if Vg-like proteins can regulate lifespan in insects generally, we examined the effects of expression of Apis Vg and Drosophila CG31150 (a Vg-like gene recently identified as cv-d) on Drosophila melanogaster lifespan and fecundity using the RU486-inducible GeneSwitch system. For all genotypes tested, overexpression of Vg and CG31150 decreased Drosophila lifespan and did not affect total or age-specific fecundity. We also detected an apparent effect of the GeneSwitch system itself, wherein RU486 exposure (or the GAL4 expression it induces) led to a significant increase in longevity and decrease in fecundity in our fly strains. This result is consistent with the pattern reported in a recent meta-analysis of Drosophila aging studies, where transgenic constructs of the UAS/GAL4 expression system that should have no effect (e.g. an uninduced GeneSwitch) significantly extended lifespan in some genetic backgrounds. Our results suggest that Vg-family genes are not major regulators of Drosophila life history traits, and highlight the importance of using appropriate controls in aging studies.