Lesion bypass in yeast cells:: Pol η participates in a multi-DNA polymerase process
Lesion bypass in yeast cells:: Pol η participates in a multi-DNA polymerase process
复制标题
DOI：
10.1093/emboj/cdf363
复制
发表时间：
2002-07-15
期刊：
影响因子：
11.4
通讯作者：
Fuchs, RPP
中科院分区：
文献类型：
--
作者：
Bresson, A;Fuchs, RPP
比较了通过(6-4)TT和G-AAF损伤在熟练和缺乏RAD30编码的DNA聚合酶ETA(PolETA)的酿酒酵母菌株中的复制。在RAD30株中，(6-4)TT损伤被错误复制和准确复制的比例分别为60%和40%。令人惊讶的是，在rad30Delta菌株中，突变旁路的水平基本上被抑制，而无错误的旁路保持不变。因此，POL ETA负责通过(6-4)TT光产物进行突变复制，而另一个聚合酶则介导其无错误旁路。RAD30基因的缺失还可以将两种不同序列背景下AAF病变的准确和不准确旁路水平降低多达8倍。这些数据表明，与Pol ETA对TT环丁烷二聚体的准确旁路相反，它负责其他病变的突变旁路。综上所述，这篇论文表明，在酵母中，跨损伤合成涉及几种聚合酶的联合作用。
Replication through (6-4)TT and G-AAF lesions was compared in Saccharomyces cerevisiae strains proficient and deficient for the RAD30-encoded DNA polymerase eta (Pol eta). In the RAD30 strain, the (6-4)TT lesion is replicated both inaccurately and accurately 60 and 40% of the time, respectively. Surprisingly, in a rad30Delta strain, the level of mutagenic bypass is essentially suppressed, while error-free bypass remains unchanged. Therefore, Pol eta is responsible for mutagenic replication through the (6-4)TT photoproduct, while another polymerase mediates its error-free bypass. Deletion of the RAD30 gene also reduces the levels of both accurate and inaccurate bypass of AAF lesions within two different sequence contexts up to 8-fold. These data show that, in contrast to the accurate bypass by Pol eta of TT cyclobutane dimers, it is responsible for the mutagenic bypass of other lesions. In conclusion, this paper shows that, in yeast, translesion synthesis involves the combined action of several polymerases.