STUDY ON LIGHT AGING OF SILK FABRIC BY FOURIER TRANSFORM INFRARED SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS
STUDY ON LIGHT AGING OF SILK FABRIC BY FOURIER TRANSFORM INFRARED SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS
复制标题
丝绸织物光老化的傅里叶变换红外光谱研究及主成分分析
DOI：
10.1080/00032719.2012.673098
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发表时间：
2012-01-01
影响因子：
2
通讯作者：
Du, Yiping
中科院分区：
文献类型：
--
作者：
Luo, Xiyun;Wu, Jingqing;Du, Yiping
利用傅里叶变换红外光谱技术(FTIR/ATR)对蚕丝老化过程进行了分析，研究了环境对蚕丝老化过程的影响。研究了真丝织物经紫外线降解后化学结构和构象结构的变化。对于重叠的酰胺III区域，原始红外光谱和二阶导数红外光谱都表明，紫外光照射诱导了结构从β-折叠结构域到β-转角构象的转变，这是由与聚甘氨酸相关的1015 cm(-1)的β-转角标记带的增加所支持的。此外，(GlyAla)(N)片段在1000和976 cM(-1)处的还原条带以及与β-折叠结构相关的1070 cM(-1)处的还原条带也说明了构象转变。此外，我们应用主成分分析的化学计量学方法，基于酰胺I光谱区的变化来评估丝绸的降解。利用前两个主成分得到的积分图有效地概括了蚕丝老化的总体趋势，并揭示了蚕丝在老化初期(12h和24h)和108小时阶段出现了快速降解。这项工作为红外光谱在丝绸降解的非侵入性定性和定量研究中提供了一种潜在的新的应用。
To investigate environmental effect on cultural materials, Fourier transform infrared technique (FTIR/ATR) was applied to analyze the silk aging process. Changes in chemical and conformational structures of silk fabric undergoing ultraviolet induced degradation were investigated. The IR spectra provided strong bands of photosensitive amide I, II, and III. For overlapping amide III region, both of the original and second-derivative IR spectra revealed that UV irradiation induced the structural transformation from beta-sheet domain to beta-turn conformation, which was supported by the increase of beta-turn marker band at 1015 cm(-1) associated with polyglycine. Additionally the reduced bands associated with (GlyAla)(n) segment at 1000 and 976 cm(-1), and the decreased band at 1070 cm(-1) related to beta-sheet structure also illustrated the conformational transition. Furthermore, we applied a chemometric method of principal component analysis for the evaluation of silk degradation based on variations in amide I spectral region. The score plot using the first two principal components effectively summarized the general trend of silk-aging and revealed the quick degradation appeared in the initial stages (12 h and 24 h) and the 108-hr stage. This work provides a potential novel application of the infrared spectroscopy in noninvasive qualitative and quantitative investigation of silk degradation.